Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Ras Gene

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

Cancer-Critical Genes I: Proto-oncogenes

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

Cancer-Critical Genes I: Proto-oncogenes

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The National Pancreatic Cancer Audit: Reducing Variation in Clinical Practice.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026

Same author

Reducing Variation in Palliative Treatment for Oesophago-Gastric Cancer: Insights from the National Oesophago-Gastric Cancer Audit.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026

Same author

MacroH2A1.1 as a crossroad between epigenetics, inflammation and metabolism of mesenchymal stromal cells in myelodysplastic syndromes.

Cell death & disease·2023

Same author

Variation in Rates of Post-Mastectomy Radiotherapy Among Women with Early Invasive Breast Cancer in England and Wales: A Population-Based Cohort Study.

Clinical oncology (Royal College of Radiologists (Great Britain))·2023

Same author

Single-incision robotic colorectal surgery with the da Vinci SP® surgical system: initial results of 50 cases.

Techniques in coloproctology·2023

Same author

The Association Between Survival and Receipt of Post-mastectomy Radiotherapy According to Age at Diagnosis Among Women With Early Invasive Breast Cancer: A Population-Based Cohort Study.

Clinical oncology (Royal College of Radiologists (Great Britain))·2023

Search research articles

Related Experiment Video

Updated: May 27, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

eIF5A isoforms and cancer: two brothers for two functions?

M Caraglia¹, M H Park, E C Wolff

¹Department of Biochemistry and Biophysics, Second University of Naples, Via Costantinopoli, 16, 80138 Naples, Italy. michele.caraglia@unina2.it

|December 6, 2011

Summary

Eukaryotic translation initiation factor 5A (eIF5A) regulates cell proliferation and apoptosis. Its isoforms, eIF5A-1 and eIF5A-2, have distinct roles, with eIF5A-2 potentially acting as an oncogene in cancer.

More Related Videos

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

Xenopus laevis as a Model to Identify Translation Impairment

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

Related Experiment Videos

Last Updated: May 27, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

Xenopus laevis as a Model to Identify Translation Impairment

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

Eukaryotic translation initiation factor 5A (eIF5A) is unique for containing the amino acid hypusine.
Vertebrates possess two eIF5A genes (eIF5A-1 and eIF5A-2) with distinct expression patterns and potential functions.

Purpose of the Study:

To review the role of hypusine formation in eIF5A in regulating cell proliferation and apoptosis.
To explore the distinct biological functions of eIF5A-1 and eIF5A-2 isoforms.
To investigate the potential oncogenic role and prognostic significance of eIF5A-2 in cancer.

Main Methods:

Literature review focusing on eIF5A function, hypusine formation, and isoform-specific roles.
Analysis of expression patterns of eIF5A-1 and eIF5A-2 in various cell types and cancer tissues.
Review of studies linking eIF5A isoforms to cell proliferation, apoptosis, and cancer prognosis.

Main Results:

eIF5A-1 is widely expressed and involved in triggering apoptosis and regulating cell proliferation.
eIF5A-2 is selectively detected in certain human cancers, suggesting an oncogenic role.
eIF5A-2 expression correlates with patient survival, indicating prognostic significance.

Conclusions:

eIF5A-1 plays a crucial role in fundamental cellular processes like proliferation and apoptosis.
eIF5A-2 may function as an oncogene and serves as a potential prognostic marker in cancer.
Both eIF5A isoforms represent potential molecular targets for anti-cancer therapies and diagnostic markers.