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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Types of RNA01:20

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Published on: May 24, 2017

Progress in studies on the RNA world.

Prakash C Joshi1, Michael F Aldersley, Jonathan D Price

  • 1Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.

Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life
|December 6, 2011
PubMed
Summary
This summary is machine-generated.

Montmorillonite clay catalyzes the formation of RNA-like chains from ImpA and ImpU. This process favors homochiral products, with chirality increasing significantly with chain length, suggesting a mechanism for prebiotic RNA replication.

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Area of Science:

  • Prebiotic chemistry and origin of life studies
  • Clay catalysis and mineral surface reactions
  • Nucleic acid chemistry and polymer formation

Background:

  • The formation of RNA oligomers is a critical step in understanding the origin of life.
  • Mineral catalysts, such as montmorillonite, are proposed to have played a role in prebiotic synthesis.
  • Understanding the factors influencing RNA polymerization, including chirality, is essential for origin-of-life scenarios.

Purpose of the Study:

  • To investigate the montmorillonite-catalyzed formation of RNA-like oligomers from ImpA and ImpU.
  • To analyze the structures and homochirality of oligomers ranging from dimers to pentamers.
  • To explore the influence of reaction conditions (pH, cation type, interlayer occupancy) on catalysis.

Main Methods:

  • Montmorillonite-catalyzed reactions of D, L-ImpA and D, L-ImpU.
  • Structural analysis of RNA-like oligomers (dimers to pentamers).
  • Investigation of reaction parameters: pH, cation influence, and interlayer space occupancy.

Main Results:

  • RNA-like oligomers were generated, with homochiral products formed in higher yields than expected statistically.
  • Homochirality increased from 64% in dimers to 97% in pentamers.
  • Detailed analysis of dimer intermediates and synthesis of non-standard dimers, including deoxy-ribonucleotides, were performed.

Conclusions:

  • Montmorillonite effectively catalyzes the formation of RNA-like oligomers with increasing homochirality.
  • The observed increase in homochirality with chain length suggests a mechanism for chiral selection in prebiotic RNA synthesis.
  • Reaction conditions significantly influence the catalytic process, providing insights into the prebiotic formation of functional nucleic acids.