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Updated: May 26, 2026

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Published on: March 23, 2011

Age-associated decrease of SIRT1 expression in rat hippocampus: prevention by late onset caloric restriction.

Ana Quintas¹, Alain J de Solís, F Javier Díez-Guerra

¹Centro de Biología Molecular Severo Ochoa, UAM-CSIC, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain.

Experimental Gerontology

|December 7, 2011

Summary

Aging reduces SIRT1 protein in rat hippocampus, but late-onset caloric restriction (CR) prevents this decline. Old rats can still benefit from CR, suggesting post-transcriptional regulation of SIRT1.

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Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice

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Published on: July 2, 2020

Area of Science:

Neuroscience
Aging Research
Molecular Biology

Background:

Sirtuin 1 (SIRT1) is a key protein deacetylase involved in cellular regulation.
Aging is associated with reduced SIRT1 levels in brain regions like the hippocampus.
Caloric restriction (CR) is known to have beneficial effects on aging, potentially mediated by SIRT1.

Purpose of the Study:

To investigate the impact of aging and late-onset caloric restriction (CR) on SIRT1 expression in the rat hippocampus and cerebral cortex.
To determine if CR can mitigate age-related decreases in SIRT1 protein levels.
To explore the role of post-transcriptional mechanisms in regulating SIRT1 expression during aging and CR.

Main Methods:

Quantitative analysis of SIRT1 protein levels in hippocampus and cerebral cortex of aged and young rats.
Immunohistochemical examination of SIRT1 expression in coronal brain sections.
Analysis of SIRT1 transcript levels to assess transcriptional regulation.

Main Results:

Aging significantly reduced SIRT1 protein levels in the rat hippocampus.
Late-onset, moderate CR effectively prevented the age-related decline in hippocampal SIRT1 protein.
SIRT1 immunoreactivity in the hippocampus confirmed that aged rats responded to CR.
No significant differences in SIRT1 transcript levels were observed among the groups.

Conclusions:

Aging negatively impacts SIRT1 protein levels in the hippocampus.
Late-onset caloric restriction can counteract age-related reductions in SIRT1 protein.
Post-transcriptional mechanisms likely play a crucial role in the regulation of SIRT1 expression by aging and CR.