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Updated: May 26, 2026
Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
Published on: May 6, 2018
In vitro Enterococcus faecalis biofilm formation on five adhesive systems
Pilar Baca1, Márcia Furtado-Antunes de Freitas, Carmen-María Ferrer-Luque
1Department of Preventive and Operative Dentistry, School of Dentistry. University of Granada, Granada, Spain.
Objective:
To determine the E. faecalis biofilm formation on the surface of five adhesive systems (AS) and its relationship with roughness.
Study Design:
The formation of E. faecalis biofilms was tested on the surface of four dual-cure AS: AdheSE DC, Clearfil DC Bond, Futurabond DC and Excite DSC and one light-cure antimicrobial AS, Clearfil Protect Bond, after 24 hours of incubation, using the MBEC high-throughput device.
Results:
E. faecalis biofilms grew on all the adhesives. The least growth of biofilm was on Excite DSC, Clearfil Protect Bond, and the control. Futurabond DC resulted in the greatest roughness and biofilm amount. There was a close relationship between the quantity of biofilm and roughness, except for Clearfil Protect Bond, which showed little biofilm but high roughness.
Conclusion:
None of the tested AS prevented E. faecalis biofilm formation, although the least quantity was found on the surface of Clearfil Protect Bond.
