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Published on: May 6, 2018
In vitro Enterococcus faecalis biofilm formation on five adhesive systems
Pilar Baca1, Márcia Furtado-Antunes de Freitas, Carmen-María Ferrer-Luque
1Department of Preventive and Operative Dentistry, School of Dentistry. University of Granada, Granada, Spain.
Medicina Oral, Patologia Oral Y Cirugia Bucal
|December 7, 2011
Summary
None of the tested dental adhesive systems (AS) completely prevented Enterococcus faecalis biofilm formation. However, Clearfil Protect Bond showed the least biofilm growth, despite some surface roughness.
Area of Science:
- Dental Materials Science
- Microbiology
- Biomaterials
Background:
- Dental adhesive systems (AS) are crucial in restorative dentistry.
- Biofilm formation on dental materials can lead to secondary caries and treatment failure.
- Enterococcus faecalis is a common pathogen implicated in endodontic infections and biofilm formation.
Purpose of the Study:
- To evaluate Enterococcus faecalis biofilm formation on five different adhesive systems.
- To investigate the relationship between surface roughness and E. faecalis biofilm accumulation on these AS.
Main Methods:
- Five adhesive systems (four dual-cure, one light-cure antimicrobial) were tested.
- E. faecalis biofilm formation was assessed after 24-hour incubation using the MBEC high-throughput device.
- Surface roughness of the AS was measured and correlated with biofilm quantity.
Main Results:
- E. faecalis biofilms formed on all tested adhesive systems.
- Excite DSC and Clearfil Protect Bond exhibited the least biofilm growth, similar to the control.
- Futurabond DC showed the highest surface roughness and the greatest amount of biofilm.
- A strong correlation between roughness and biofilm quantity was observed, with an exception for Clearfil Protect Bond.
Conclusions:
- No tested adhesive system completely inhibited E. faecalis biofilm formation.
- Clearfil Protect Bond demonstrated the lowest E. faecalis biofilm accumulation among the evaluated AS.
- Surface properties, particularly roughness, play a significant role in E. faecalis biofilm adherence to dental adhesives.
