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Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
Published on: March 31, 2008
Labeling of multiple cell lines using a new iron oxide agent for cell tracking by MRI
C McFadden1, C L Mallett, P J Foster
1Imaging Research Laboratory, Robarts Research Institute, The University of Western Ontario, London, ON, Canada.
Contrast Media & Molecular Imaging
|December 7, 2011
Summary
A novel nanoparticle, Molday ION Rhodamine-B (MIRB), effectively labels stem cells, cancer cells, and immune cells. This allows for in vivo detection using magnetic resonance imaging (MRI).
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Cell Biology
Background:
- Cellular labeling is crucial for tracking cells in vivo.
- Existing methods may affect cell viability or function.
- Need for efficient, non-toxic labeling agents for various cell types.
Purpose of the Study:
- To evaluate the efficacy of Molday ION Rhodamine-B (MIRB) for labeling diverse cell types.
- To assess the impact of MIRB labeling on cell viability and function.
- To determine the suitability of MIRB for in vivo imaging via MRI.
Main Methods:
- Co-incubation of stem cells, cancer cells, and immune cells with MIRB.
- Assessment of labeling efficiency using iron staining and fluorescence imaging.
- Evaluation of cell viability and function through flow cytometry.
- Transmission electron microscopy for ultrastructural analysis.
- In vitro testing for potential in vivo magnetic resonance imaging (MRI) at 3 Tesla.
Main Results:
- MIRB successfully labeled both adherent and nonadherent cell lines.
- High cell viability was maintained post-labeling.
- Sufficient MIRB loading levels were achieved for detection.
- Demonstrated potential for in vivo MRI detection at 3 T.
Conclusions:
- MIRB is a versatile and effective nanoparticle for labeling various cell types.
- MIRB labeling preserves cell viability and function.
- MIRB holds promise for non-invasive cell tracking using MRI.
