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Related Experiment Video

Updated: May 26, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Microfluidic primer extension assay.

Markus Beier1, Valesca Boisguérin

  • 1Febit Biomed Gmbh, Heidelberg, Germany. markus.beier@febit.de

Methods in Molecular Biology (Clifton, N.J.)
|December 7, 2011
PubMed
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This study presents a simple method for detecting microRNAs (miRNAs) in body fluids using microfluidic microarrays. The technique enables sensitive identification of miRNA signatures for disease diagnosis from minimal total RNA samples.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are crucial noncoding RNA regulators of gene expression.
  • miRNAs are increasingly recognized as biomarkers, found in tissues and body fluids.
  • Detecting short, homologous miRNAs presents significant analytical challenges.

Purpose of the Study:

  • To develop a simple, efficient, and specific method for miRNA detection.
  • To enable miRNA analysis directly from total RNA without prior enrichment or labeling.
  • To establish a tool for identifying disease-specific miRNA signatures.

Main Methods:

  • Utilized stringent hybridization and enzymatic primer extension on a microfluidic microarray.
  • Developed an assay starting from total RNA material, requiring only 30 ng.

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Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
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Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
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A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Related Experiment Videos

Last Updated: May 26, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
15:28

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

  • Applied the method for high-throughput analysis of known miRNAs.

    • Main Results:

    • Demonstrated a simple method for miRNA detection from total RNA.
    • The assay is sensitive, requiring minimal starting material (30 ng).
    • Successfully identified miRNA signatures for diagnosing cancer and cardiovascular/inflammatory diseases.

    Conclusions:

    • The described method offers a straightforward approach for miRNA detection and signature identification.
    • This technique facilitates biomarker discovery and disease diagnostics using body fluid samples.
    • The assay's simplicity and low input requirement make it valuable for clinical applications.