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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

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Published on: August 21, 2019

MicroRNA expression analysis using the Illumina microRNA-Seq Platform.

¹Illumina, Inc., Hayward, CA, USA. sluo@illumina.com

Methods in Molecular Biology (Clifton, N.J.)

|December 7, 2011

Summary

Next-generation sequencing (NGS) enables direct RNA sequencing for transcriptome analysis. Illumina's microRNA-Seq method allows for identifying and profiling microRNAs, aiding in understanding their biogenesis and modification.

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Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Next-generation sequencing (NGS) has transformed transcriptome analysis with high throughput and reduced costs.
Direct RNA sequencing offers a powerful approach to studying RNA molecules.

Purpose of the Study:

To describe Illumina's microRNA-Seq method for sequencing microRNAs.
To highlight the utility of this method for identifying and profiling microRNAs across organisms.
To provide insights into microRNA biogenesis and modification.

Main Methods:

Utilizing the Illumina Genome Analyzer system for microRNA sequencing.
Direct sequencing of RNA molecules.

Main Results:

The microRNA-Seq method generates sequence data for direct microRNA identification and profiling.
The approach is applicable to microRNAs from any organism.

Conclusions:

Illumina's microRNA-Seq is a valuable tool for comprehensive microRNA analysis.
This method advances the understanding of microRNA biogenesis and modification processes.