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Updated: May 26, 2026
Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
Published on: September 11, 2014
Biochemical characterization of a testis-predominant isoform of N-alpha acetyltransferase
1Section on Clinical Genomics, Laboratory of Clinical and Developmental Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA. panga@mail.nih.gov
Abstract:
N-alpha protein acetylation, catalyzed by N-alpha acetyltransferase complex, is a common protein -modification process in eukaryotic cells. Despite its widespread occurrence, the biological significance of this modification process is still unclear. We recently discovered a novel testis-predominant isoform of the catalytic subunit of the enzyme complex. Here, we describe the biochemical characterization of this testis-predominant N-alpha acetyltransferase complex, which includes protein-protein interaction study by co-immunoprecipitation experiment and functional study by N-alpha acetyltransferase assay.
