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Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
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Posttranslational lipid modification of Rho family small GTPases

Natalia Mitin1, Patrick J Roberts, Emily J Chenette

  • 1Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA. natalia_mitin@med.unc.edu

Methods in Molecular Biology (Clifton, N.J.)
|December 7, 2011
PubMed
Summary

Rho GTPases are inactive proteins modified by lipids for cellular targeting. This review covers methods to study how these posttranslational lipid modifications impact Rho GTPase function.

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