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Updated: May 26, 2026

RhoC GTPase Activation Assay
Published on: August 22, 2010
Rho GTPases and cancer cell transendothelial migration
Nicolas Reymond1, Philippe Riou, Anne J Ridley
1Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.
Abstract:
Small Rho GTPases are major regulators of actin cytoskeleton dynamics and influence cell shape and migration. The expression of several Rho GTPases is often up-regulated in tumors and this frequently correlates with a poor prognosis for patients. Migration of cancer cells through endothelial cells that line the blood vessels, called transendothelial migration or extravasation, is a critical step during the metastasis process. The use of siRNA technology to target specifically each Rho family member coupled with imaging techniques allows the roles of individual Rho GTPases to be investigated. In this chapter we describe methods to assess how Rho GTPases affect the different steps of cancer cell transendothelial cell migration in vitro.
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