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Updated: May 26, 2026

RhoC GTPase Activation Assay
Published on: August 22, 2010
Functional analysis of Rho GTPase activation and inhibition in a bead-based miniaturized format
Michael Schmohl1, Stefanie Rimmele, Peter Gierschik
1Department of Biochemistry, NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany.
Abstract:
Extensive knowledge about protein-protein interactions is fundamental to fully understand signaling pathways and for the development of new drugs. Rho GTPases are key molecules in cellular signaling processes and their deregulation is implicated in the development of a variety of diseases such as neurofibromatosis type 2 and cancer. Here, we describe a bead-based protein-protein interaction assay for overexpressed HA-tagged Rho GTPases to study the GTPγS-dependent interaction with the regulatory protein RhoGDIα. This assay provides a useful tool for the analysis of both macromolecular and small molecule activators and inhibitors of the protein-protein interactions of Rho GTPases with their regulatory proteins in a multiplexed miniaturized format.
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