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Updated: May 26, 2026

RhoC GTPase Activation Assay
Published on: August 22, 2010
Analysis of Rho GTPase activation in Saccharomyces cerevisiae
1Department of Cell Biology, University of Alberta, Edmonton, AB, Canada. gary.eitzen@ualberta.ca
Abstract:
Rho proteins act as molecular switches to control multiple cellular processes. The switch mechanism involves cycling between active and inactive states based on GTP loading and hydrolysis. Assays that quantitatively analyze the GTP loading of Rho proteins have become important molecular tools to decipher upstream signals and mechanisms that regulate activation and de-activation. These assays make use of Rho activation probes constructed from Rho-binding domains of downstream effectors. The utility of these assays comes from effector domains that show selective high affinity interactions with specific subsets of GTP-bound activated GTPases. Here, we describe assays used to analyze yeast Rho GTPase activation.
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