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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

siRNA - Small Interfering RNAs

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Leaky Scanning

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Updated: May 26, 2026

RNA Interference in Ticks

RNA Interference in Ticks

Published on: January 20, 2011

Scavenger receptor mediates systemic RNA interference in ticks.

Kyaw Min Aung¹, Damdinsuren Boldbaatar, Rika Umemiya-Shirafuji

¹Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi, Japan.

|December 7, 2011

Summary

Class B scavenger receptor CD36 (SRB) mediates RNA interference in ticks. SRB dsRNA injection effectively silences target genes and impacts tick engorgement, mortality, and hatchability, revealing its crucial role in systemic RNAi.

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Last Updated: May 26, 2026

RNA Interference in Ticks

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Published on: January 20, 2011

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles

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Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

Published on: October 13, 2014

Area of Science:

Entomology
Molecular Biology
Genetics

Background:

RNA interference (RNAi) is a powerful tool for gene silencing.
Class B scavenger receptor CD36 (SRB) is implicated in cellular processes.
Understanding gene regulation in ticks is crucial for controlling disease transmission.

Purpose of the Study:

To investigate the role of H. longicornis SRB (HlSRB) in mediating RNAi responses in ticks.
To determine if HlSRB can facilitate systemic gene silencing.
To assess the impact of HlSRB-mediated RNAi on tick physiology and reproduction.

Main Methods:

Injection of double-stranded RNA (dsRNA) targeting HlSRB, HlVg-1, and HlVgR in unfed female H. longicornis ticks.
Assessment of gene silencing efficacy through specific and systemic knockdown.
Phenotypic analysis of engorgement, mortality, and hatchability.
Western blot and immunohistochemical analyses to confirm protein expression changes.

Main Results:

Specific and systemic gene silencing of HlSRB, HlVg-1, and HlVgR was achieved with single dsRNA injections.
Sequential injection of HlVg-1/HlSRB or HlVgR/HlSRB dsRNA resulted in successful double knockdown and significant phenotypic changes.
Pre-silencing of HlSRB inhibited subsequent RNAi of HlVg-1 and HlVgR, indicating SRB's essential role in systemic RNAi.
Endogenous HlSRB protein was abolished, while HlVg-1 and HlVgR expression was affected by the order of dsRNA administration.

Conclusions:

Class B scavenger receptor CD36 (SRB) plays a critical role in mediating RNA interference in ticks.
SRB facilitates not only gene knockdown but also systemic RNAi responses.
The findings highlight SRB as a potential target for tick control strategies.