[Optimal signal filtering parameters for tetrode recording of neuronal activity]

Extracellular recording of neuronal spiking is the main method of investigation of involvement of neurons in behavioral tasks. Development of multichannel electrodes made it possible to simultaneously record activity of the same group of neurons from different locations in the brain tissue. That method allows the researches to distinguish spiking of simultaneously recorded neurons by individual set of projection coefficients of amplitude parameters on axes corresponding to different channels of the multichannel electrode. We tested the possibility of effective separation of single unit spiking streams from multiunit activity recorded by tetrode and subjected to different filtering. We described the main limitations for effective spike identification and determined the optimal band of signal filtering for tetrode recording.