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Updated: May 26, 2026

Forward Genetic Approaches in Chlamydia trachomatis
Published on: October 23, 2013
[Immunogenic properties and molecular-genetic features of native and inactivated Chlamydia trachomatis MT-2A
Unlabelled:
AIM. Study of antibody genesis after introduction of native and inactivated Chlamydia trachomatis MT-2A strain into Balb/c mice and analysis of molecular-genetic features of omp A gene that is responsible for its immunogenicity.
Materials And Methods:
Chlamydia infection was modeled in Balb/c mice by using human isolated C. trachomatis serovariant D.
Results:
After the introduction of this strain, 5 days after the infection of mice A, M and G class antibodies including G class antibodies against chlamydia heat shock protein are induced. Studies of molecular-genetic features of the isolated C. trachomatis MT-2A strain showed presence of 2 nucleotide mutations in ompAgene. A synonymous substitution of cytosine to thymine in position 574 (CD) that does not result in amino acid substitution in the protein was registered. A non-synonymous substitution of thymine to guanine was present in position 843 (VDIII), that is most probably one of the main reasons of the registered higher immunogenicity of C. trachomatis MT-2A serovariant D strain.
Conclusion:
Further studies of C. trachomatis MT-2A serovariant D strain are promising, but do not exclude search of other immunogenic strains for the generation of agents for specific prophylaxis and immunotherapy of chlamydiosis.
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