[VEGF gene expression in transfected human multipotent stromal cells].

Area of Science:

• Cell biology
• Gene therapy
• Molecular biology

Context:

• Adipose-derived multipotent stromal cells (MSCs) are a promising source for regenerative medicine.
• Efficient and stable gene expression in MSCs is crucial for therapeutic applications.
• Nonviral transfection methods offer potential advantages over viral vectors.

Purpose:

• To investigate the dynamics of vascular endothelial growth factor (VEGF) gene expression in adipose-derived MSCs following nonviral transfection.
• To compare the efficacy of electroporation and lipofection in terms of transfection efficiency, plasmid elimination, and VEGF expression levels.
• To identify factors influencing gene expression stability and magnitude in transfected MSCs.

Summary:

• Electroporation and lipofection were employed to transfect adipose-derived MSCs with a VEGF-expressing plasmid.
• Distinct patterns of plasmid elimination were observed, categorizing cultures into fast and slow eliminators.
• Significant interculture variability in VEGF expression was detected, with a potential for 5-6 fold increase.
• No significant differences were found between electroporation and lipofection regarding transfection potency, plasmid elimination dynamics, or VEGF expression.

Impact:

• This study provides insights into the variability of gene expression following nonviral transfection of MSCs.
• Understanding plasmid elimination dynamics is essential for optimizing gene therapy strategies using MSCs.
• The findings highlight the potential for achieving substantial VEGF expression increases, relevant for angiogenic therapies.