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Related Experiment Video

Updated: May 26, 2026

CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells
10:59

CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells

Published on: October 18, 2014

Single cell DNA damage/repair assay using HaloChip.

Yong Qiao1, Chaoming Wang, Ming Su

  • 1NanoScience Technology Center, University of Central Florida, Orlando, Florida 32826, United States.

Analytical Chemistry
|December 8, 2011
PubMed
Summary
This summary is machine-generated.

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A new HaloChip assay detects DNA damage sensitively at the single-cell level. This high-throughput method automates analysis of millions of cells, improving DNA damage detection efficiency.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • DNA is susceptible to free radical damage, impacting cell function.
  • Existing DNA damage detection methods lack sensitivity, throughput, and automation.
  • Need for advanced assays to accurately quantify DNA damage.

Purpose of the Study:

  • Develop a novel cell array-based assay for sensitive DNA damage detection.
  • Enable high-throughput and automated analysis of DNA damage at the single-cell level.
  • Overcome limitations of current DNA damage detection techniques.

Main Methods:

  • Developed HaloChip assay using electrostatic interactions to pattern mammalian cells on microfabricated surfaces.
  • Trapped cells in gels, allowing damaged DNA fragments to form halos around cells.

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Last Updated: May 26, 2026

CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells
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Published on: October 18, 2014

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  • Utilized ethidium bromide staining and fluorescence microscopy for halo visualization.
  • Implemented spatial encoding for multiple dosing conditions on a single chip.

    • Main Results:

    • Achieved sensitive and accurate DNA damage detection at the single-cell level via halo formation.
    • Demonstrated automated analysis of millions of cells without overlapping issues.
    • Successfully detected DNA damage induced by chemicals, UV, and X-ray irradiation.
    • Enabled measurement of cellular response variations to DNA damaging agents.

    Conclusions:

    • HaloChip offers a highly efficient, sensitive, and automated method for DNA damage assessment.
    • The assay significantly improves throughput and analytical capabilities compared to existing methods.
    • HaloChip is applicable to diverse settings including clinical, epidemiological, and experimental research.