Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Testosterone: Functions and Regulation

Testosterone: Functions and Regulation

The intricate hormonal interplay essential for male reproductive health begins with the release of gonadotropin-releasing hormone (GnRH) by the hypothalamus. This hormone prompts the pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). LH targets the Leydig cells in the testes, stimulating them to produce and release testosterone. In concert with testosterone, FSH acts on the Sertoli cells within the seminiferous tubules to facilitate the release of...

Hormonal Regulation of the Menstrual Cycle

Hormonal Regulation of the Menstrual Cycle

The ovarian cycle regulates endometrial changes throughout a single menstrual cycle via the coordinated action of gonadotrophin-releasing hormone (GnRH) and gonadotrophins.
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH release.

Feedback Regulation of Calcium Concentration

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...

Hormonal Control of the Ovarian Cycle

Hormonal Control of the Ovarian Cycle

The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...

Hormones of the Pituitary Gland

Hormones of the Pituitary Gland

The small, pea-sized pituitary gland is located at the base of the brain. It is crucial in regulating various bodily functions, from growth to reproduction. The gland is divided into the anterior lobe and the posterior lobe. The secretory cell clusters in the pars distalis of the anterior pituitary lobe are controlled by hypothalamic regulators and synthesize six primary hormones.
The most abundantly secreted hormone from the anterior lobe is the growth hormone, which controls overall growth by...

Regulation of Hormone Secretion

Regulation of Hormone Secretion

Regulation of hormone secretion is a finely tuned orchestration driven by various types of stimuli, encompassing neural, humoral, and hormonal signals. Environmental cues instigate neural stimuli, where action potentials traverse nerve fibers to reach their designated targets. An illustrative scenario is the body's response to stress, wherein the sympathetic nervous system releases epinephrine from the adrenal glands, inducing the well-known 'fight or flight' reaction.
Humoral stimuli,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Single and multi-dose pharmacology of recombinant and urinary human chorionic gonadotrophin in men.

Clinical endocrinology·2024

Same author

Prevalence of nandrolone preparations with endogenous carbon isotope ratios in Australia.

Drug testing and analysis·2024

Same author

Summer Olympic sports and female athletes: comparison of anti-doping collections and prohibited substances detected in Australia and New Zealand vs. France.

Frontiers in sports and active living·2023

Same author

Reliability of Drug History to Verify Androgen Abuse in Men.

The Journal of clinical endocrinology and metabolism·2022

Same author

A multi-parametric approach to remove the influence of plasma volume on the athlete biological passport during a Union Cycliste Internationale cycling stage race.

Drug testing and analysis·2020

Same author

Ecdysteroids as non-conventional anabolic agent: performance enhancement by ecdysterone supplementation in humans.

Archives of toxicology·2019

Search research articles

Related Experiment Video

Updated: May 26, 2026

Remote Neuronal Activation Coupled with Automated Blood Sampling to Induce and Measure Circulating Luteinizing Hormone in Mice

Remote Neuronal Activation Coupled with Automated Blood Sampling to Induce and Measure Circulating Luteinizing Hormone in Mice

Published on: August 25, 2023

Stimulating luteinizing hormone.

Catrin Goebel¹

¹National Measurement Institute, Pymble, NSW, Australia. catrin.goebel@measurement.gov.au

Drug Testing and Analysis

|December 8, 2011

Summary

This summary is machine-generated.

Low molecular weight luteinizing hormone (LMWLH) receptor agonists are potential doping agents. A new liquid chromatography-tandem mass spectrometry method can detect these compounds directly in urine samples.

More Related Videos

Demonstrating a Linear Relationship Between Vascular Endothelial Growth Factor and Luteinizing Hormone in Kidney Cortex Extracts

Demonstrating a Linear Relationship Between Vascular Endothelial Growth Factor and Luteinizing Hormone in Kidney Cortex Extracts

Published on: January 22, 2020

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

Published on: July 4, 2018

Related Experiment Videos

Last Updated: May 26, 2026

Remote Neuronal Activation Coupled with Automated Blood Sampling to Induce and Measure Circulating Luteinizing Hormone in Mice

Remote Neuronal Activation Coupled with Automated Blood Sampling to Induce and Measure Circulating Luteinizing Hormone in Mice

Published on: August 25, 2023

Demonstrating a Linear Relationship Between Vascular Endothelial Growth Factor and Luteinizing Hormone in Kidney Cortex Extracts

Demonstrating a Linear Relationship Between Vascular Endothelial Growth Factor and Luteinizing Hormone in Kidney Cortex Extracts

Published on: January 22, 2020

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

Frequent Tail-tip Blood Sampling in Mice for the Assessment of Pulsatile Luteinizing Hormone Secretion

Published on: July 4, 2018

Area of Science:

Sports Science
Analytical Chemistry
Endocrinology

Background:

Low molecular weight luteinizing hormone (LMWLH) receptor agonists are orally active compounds that stimulate endogenous hormone production, mimicking Luteinizing Hormone (LH).
These substances are of interest as potential doping agents in sports due to their anabolic effects.
Direct detection methods are necessary as indirect analysis of endogenous hormone levels is insufficient to prove their use.

Purpose of the Study:

To develop and validate a direct detection method for LMWLH receptor agonists in athlete urine samples.
To establish a screening methodology for a broad range of potential LMWLH receptor agonists.
To assess the suitability of the developed method for general doping control screening.

Main Methods:

Development of a precursor ion monitoring liquid chromatography tandem mass spectrometry (LC-MS/MS) method.
Extraction of selected LMWLH receptor agonists from urine using established methodologies.
Testing the method against urine samples to identify potential interferences from background analytes.

Main Results:

The developed LC-MS/MS method successfully detected two available LMWLH receptor agonists.
Detection limits of 100 ng/ml were achieved in spiked urine samples.
The method demonstrated suitability for screening purposes, with considerations for background analyte interference.

Conclusions:

A basic precursor ion monitoring LC-MS/MS method has been established for the direct detection of LMWLH receptor agonists in urine.
This method is suitable for screening purposes in anti-doping laboratories.
Further refinement may be needed to address potential background interferences in routine screening.