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Updated: May 26, 2026

Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models

Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models

Published on: July 7, 2016

The mouse lymphoma assay.

Melvyn Lloyd¹, Darren Kidd

¹Covance Laboratories Limited, Harrogate, UK. mel.lloyd@covance.com

Methods in Molecular Biology (Clifton, N.J.)

|December 8, 2011

Summary

This summary is machine-generated.

The mouse lymphoma TK assay detects gene and chromosomal mutations in mouse cells using triflurothymidine. This in vitro test aids in predicting potential risks before animal studies.

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Area of Science:

Toxicology
Genetics

Background:

The mouse lymphoma TK assay (MLA) is an in vitro test for risk assessment.
It is part of a battery of tests preceding in vivo studies.

Purpose of the Study:

To detect mutagenic and clastogenic events at the thymidine kinase (tk) locus.
To measure resistance to triflurothymidine (TFT) in L5178Y mouse lymphoma cells.

Main Methods:

Cells are plated in agar or microwell assays for viability and mutation detection.
Mutant frequency is determined by culturing cells in selective medium containing TFT.
Colony size differentiates between gene mutations (large) and chromosomal mutations (small).

Main Results:

Wild-type cells (tk +/-) die in TFT medium.
Mutant cells (tk -/-) survive and form colonies.
Mutant frequency is reported as mutants per 10(6) viable cells.

Conclusions:

The MLA assay can distinguish between gene and chromosomal mutations.
It serves as a valuable tool in genotoxicity testing and risk assessment.