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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: May 26, 2026

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
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Next-generation sequencing for cancer diagnostics: a practical perspective.

Cliff Meldrum1, Maria A Doyle, Richard W Tothill

  • 1Molecular Pathology, Hunter Area Pathology Service, Newcastle, NSW 2310;

The Clinical Biochemist. Reviews
|December 8, 2011
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing (NGS) offers rapid, cost-effective genome analysis for biological research and medical diagnostics. This technology is transforming cancer research by enabling large-scale mutation cataloging for new diagnostic and therapeutic targets.

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Last Updated: May 26, 2026

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Published on: October 18, 2013

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Next-generation sequencing (NGS) represents a major technological leap in biological sciences.
  • Rapid advancements in sequencing platforms allow simultaneous sequencing of multiple genomes.
  • Targeted DNA enrichment enhances throughput and reduces per-sample costs.

Purpose of the Study:

  • To review the current state of NGS technology, methods, and applications.
  • To highlight immediate opportunities for implementing NGS in clinical settings.
  • To discuss challenges associated with the routine diagnostic use of NGS.

Main Methods:

  • Review of existing literature and technological advancements in NGS.
  • Analysis of applications in medical research, particularly in cancer genomics.
  • Discussion of practical considerations for clinical implementation.

Main Results:

  • NGS platforms are capable of sequencing multiple genomes rapidly and cost-effectively.
  • Medical research, especially cancer studies, is a primary beneficiary of NGS.
  • NGS is nearing readiness for routine diagnostic applications due to its sensitivity, speed, and cost.

Conclusions:

  • NGS technology is maturing, presenting immediate opportunities for clinical integration.
  • The ability to perform multi-gene assays is crucial for identifying genetic cancer determinants.
  • While whole-genome sequencing is still developing, targeted NGS applications are viable for diagnostics.