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[Subpopulations of immunocompetent cells in the placenta in normal pregnancy and in EPH gestosis]
I Rudelić1, G Candussi, O Radillo
1Medicinski centar Pula Djelatnost za zdravstvenu zastitu zena.
An effort of cellular element investigations in placentas of 8 physiological pregnancies and 7 EPH gestoses is performed with the aid of specific monoclonal antibodies by the indirect immunohistochemical method. The average mean of parturient ages (primiparae and pluriparae) was 24 years in EPH gestoses. By the aid of monoclonal antibodies OKT4, OKT8, OKT11, OKT6 and OKDR, Dako-macrophage, 2H4 (Coulter) murine type, immunocompetent cells were identified in the decidua, in the stroma of chorionic villi, in the intervillous space, and in the fibrinoid masses. The semiquantitative indirect method of the immunohistochemical cellular investigation was performed by the aid of optic microscopy in 20 fields per every cutting (section) of the placental tissue. The immunocompetent active cell distribution did not reveal any special difference in the frequency of placental tissues of physiogical pregnancies and EPH gestoses, but OKT11+ cells were present in the major number in gestoses (in decidua). OKT8+ and OKDR+ cells were found in a similar number in both populations, while OKT6+ were not found at all in any investigated tissue.
An effort of cellular element investigations in placentas of 8 physiological pregnancies and 7 EPH gestoses is performed with the aid of specific monoclonal antibodies by the indirect immunohistochemical method. The average mean of parturient ages (primiparae and pluriparae) was 24 years in EPH gestoses. By the aid of monoclonal antibodies OKT4, OKT8, OKT11, OKT6 and OKDR, Dako-macrophage, 2H4 (Coulter) murine type, immunocompetent cells were identified in the decidua, in the stroma of chorionic villi, in the intervillous space, and in the fibrinoid masses. The semiquantitative indirect method of the immunohistochemical cellular investigation was performed by the aid of optic microscopy in 20 fields per every cutting (section) of the placental tissue. The immunocompetent active cell distribution did not reveal any special difference in the frequency of placental tissues of physiogical pregnancies and EPH gestoses, but OKT11+ cells were present in the major number in gestoses (in decidua). OKT8+ and OKDR+ cells were found in a similar number in both populations, while OKT6+ were not found at all in any investigated tissue.