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Updated: May 26, 2026

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))
Published on: May 4, 2020
Electron energy-loss safe-dose limits for manganese valence measurements in environmentally relevant manganese oxides
Kenneth J T Livi1, Brandon Lafferty, Mengqiang Zhu
1The High-Resolution Analytical Electron Microbeam Facility of the Integrated Imaging Center Departments of Earth and Planetary Sciences and Biology, Johns Hopkins University, Baltimore, Maryland 21218, United States. klivi@jhu.edu
Abstract:
Manganese (Mn) oxides are among the strongest mineral oxidants in the environment and impose significant influence on mobility and bioavailability of redox-active substances, such as arsenic, chromium, and pharmaceutical products, through oxidation processes. Oxidizing potentials of Mn oxides are determined by Mn valence states (2+, 3+, 4+). In this study, the effects of beam damage during electron energy-loss spectroscopy (EELS) in the transmission electron microscope have been investigated to determine the "safe dose" of electrons. Time series analyses determined the safe dose fluence (electrons/nm(2)) for todorokite (10(6) e/nm(2)), acid birnessite (10(5)), triclinic birnessite (10(4)), randomly stacked birnessite (10(3)), and δ-MnO(2) (<10(3)) at 200 kV. The results show that meaningful estimates of the mean Mn valence can be acquired by EELS if proper care is taken.
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