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Related Experiment Video

Updated: May 26, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Microbes, intestinal inflammation and probiotics.

Mohammad W Khan¹, Amod A Kale, Praveen Bere

¹The Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. mohammad-khan@northwestern.edu

Expert Review of Gastroenterology & Hepatology

|December 14, 2011

Summary

Inflammatory bowel disease (IBD) disrupts the gut's balance. This review explores how gut cells, bacteria, and immune cells interact, and if genetically modified probiotics can reduce IBD inflammation.

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Last Updated: May 26, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach

Published on: May 2, 2018

Area of Science:

Gastroenterology
Immunology
Microbiology

Background:

Inflammatory bowel disease (IBD) disrupts intestinal homeostasis.
IBD involves complex interactions between the immune system, intestinal epithelium, and gut microbiota.
Understanding these intricate relationships is crucial for developing effective treatments.

Purpose of the Study:

To review the interplay between intestinal epithelial cells, bacteria, and innate immunity in IBD.
To discuss the potential of genetically modified probiotic bacteria in mitigating IBD-related inflammation.

Main Methods:

Literature review focusing on IBD pathogenesis.
Analysis of immune responses involving macrophages, dendritic cells, and epithelial cells.
Exploration of microbial and genetic interventions for IBD.

Main Results:

Intestinal macrophages, dendritic cells, and epithelial cells form a key triad governing immune responses.
The gut microbiota plays a critical role in initiating and modulating intestinal inflammation.
Genetic modification of probiotics presents a potential therapeutic avenue for IBD.

Conclusions:

The intricate crosstalk between host and microbial factors is central to IBD.
Targeting these interactions, potentially through engineered probiotics, offers promising strategies for managing IBD.