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Related Concept Videos

iChip01:24

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Related Experiment Video

Updated: May 26, 2026

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors
12:39

Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

Published on: May 21, 2008

Phage-chips for novel optically readable tissue engineering assays.

So Young Yoo1, Jin-Woo Oh, Seung-Wuk Lee

  • 1Department of Bioengineering, and Berkeley Nanoscience and Nanoengineering Institute, University of California, Berkeley, Berkeley, California 94720, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|December 14, 2011
PubMed
Summary
This summary is machine-generated.

Novel phage-based array chips offer optical readability for cell proliferation and morphology assays. Engineered phages serve as promising coating materials for lab-on-a-chip platforms, enabling sensitive biochemical monitoring and drug discovery.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Phage display technology enables the engineering of bacteriophages for specific applications.
  • Lab-on-a-chip (LOC) platforms require advanced surface coatings for sensitive biological assays.
  • Monitoring cell proliferation and morphology is crucial for understanding cellular responses and drug efficacy.

Purpose of the Study:

  • To develop novel phage-based array chips for optically readable cell proliferation and morphology assays.
  • To engineer M13 phages displaying RGD and/or FGFb for creating functionalized nanofibrous networks.
  • To demonstrate the utility of engineered phages as coating materials for LOC platforms.

Main Methods:

  • Engineered M13 phages displaying RGD and/or FGFb were used to create phage spot matrices.
  • Self-assembled nanofibrous network structures were formed on the array chips.
  • Fibroblasts were cultured on the phage-functionalized arrays.
  • Surface plasmon resonance (SPR) spectroscopy was employed to monitor cell behavior.

Main Results:

  • The phage-based array chips were optically readable for cell proliferation and morphology assays.
  • SPR spectroscopy successfully monitored the effects of phage-displayed biochemical cues on fibroblast growth and morphology.
  • Engineered phages facilitated sensitive detection of functional peptide effects on cell growth.

Conclusions:

  • Engineered phages are effective coating materials for LOC platforms, enabling sensitive monitoring of cellular responses.
  • Phage-chips show significant potential for high-throughput biochemical screening, biosensing, and novel drug discovery.
  • This technology advances the development of integrated bioanalytical systems for various research applications.