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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Updated: May 26, 2026

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Aptamer selection by high-throughput sequencing and informatic analysis.

Shawn Hoon¹, Bin Zhou, Kim D Janda

¹Molecular Engineering Lab, Agency for Science Technology and Research, Singapore.

|December 14, 2011

Summary

This summary is machine-generated.

A new, streamlined assay enables the selection of high-affinity aptamers in a single round. This method uses high-throughput DNA sequencing and bioinformatics, reducing hands-on time and expertise needed for aptamer discovery.

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Area of Science:

Biotechnology
Molecular Biology
Genomics

Background:

Traditional aptamer selection is a multi-step, time-consuming process.
Identifying high-affinity aptamers often requires extensive optimization.
Existing methods can be resource-intensive and require specialized expertise.

Purpose of the Study:

To develop a simplified, single-round aptamer selection assay.
To enable rapid identification of high-affinity aptamers.
To make aptamer selection accessible to laboratories with limited expertise.

Main Methods:

A single round of positive selection.
High-throughput DNA sequencing.
Bioinformatic analysis for aptamer identification.

Main Results:

Successfully identified high-affinity aptamers in one selection round.
Demonstrated aptamer binding to thrombin with nanomolar dissociation constants.
The assay proved flexible and reduced hands-on time.

Conclusions:

The described assay significantly accelerates aptamer discovery.
This method lowers the barrier to entry for aptamer selection.
It offers a practical alternative for identifying potent aptamers for various targets.