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Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
Published on: November 30, 2015
WBC reduction filtration efficacy performed at varying time intervals post-collection
Ricardo Hinojosa1, Barbara J Bryant
1United Blood Services of the Rio Grande Valley, 1400 S.6th St., McAllen, TX 78501, USA. rmhinojosa@gmail.com
Transfusion
|December 14, 2011
Summary
Storage time of whole blood units before filtration does not impact white blood cell (WBC) reduction efficacy. Eliminating prefiltration storage requirements can improve blood component production efficiency.
Area of Science:
- Blood banking and transfusion medicine
- Cellular therapy and component processing
Background:
- Investigated high white blood cell (WBC) counts post-leukoreduction filtration at a blood center.
- Suspected prefiltration storage time and temperature as potential causes.
Purpose of the Study:
- To evaluate the impact of whole blood (WB) storage duration in transport shippers on residual WBC counts after leukoreduction filtration.
Main Methods:
- Collected 50 WB units using the Fenwal Express System with Integral Sepacell RZ-2000 filter.
- Stored units in shippers with ice, performing filtration at various post-collection intervals (28-458 minutes).
- Defined acceptable leukoreduction as < 5 × 10^6 residual WBC.
Main Results:
- All 50 tested WB units achieved acceptable leukoreduction.
- Residual WBC counts remained below the acceptable threshold regardless of storage duration in transport shippers.
Conclusions:
- Prefiltration storage time of WB units in transport shippers does not affect leukoreduction efficiency.
- Eliminating the mandatory 2-hour prefiltration storage can enhance blood component production laboratory efficiency and save time.
