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[The pathogenesis of CD4(+)T cells infiltrated into the spinal cord in rat SNL model]
Cai-xia Sun1, Yan-fang Liu, Cheng-lin Zhou
1Department of Immunology, Medical College of Soochow University, Suzhou 215123, China. suncaixia_zj@126.com
Aim:
To explore the infiltration pathogenesis of CD4(+);T cells following the spinal nerve ligation.
Methods:
Healthy adult male SD rats were randomly divided into the spinal nerve ligation group (Tx), sham operation group (S), control group (C). the 50& mechanical paw withdrawal threshold ( 50&MWT ) was determined by up-down method; CD4(+);T cells infiltration was assessed by FACS; the mRNA levels of CCL2, CCL5 and CXCL10 were quantitated by RT-qPCR; serum cytokines were tested by ELISA kits.
Results:
After 3 days since operation, 50&MWT of Tx group was significantly reduced (P<0.01) comparing with S group, C group; on day 14, 50&MWT was up to the minimum value; whereas S group and C group were no difference (P>0.05). After 7 days since operation, CD4(+);T cells infiltration into lumbar segments of the spinal cord in the Tx group increased significantly (P<0.01), and the CCL2, CCL5mRNA expression increased (P<0.05); on day 14, the CD4(+);T cells infiltration in Tx group was higher than S group, C group; but there was no statistical significance. On day 7 and 14 days, serum levels of cytokines were no difference in the three groups.
Conclusion:
Following spinal nerve ligation, high expression of chemokine promoted peripheral CD4(+);T cells to infiltrate into spinal cord; and the infiltrated CD4(+);T cells maintained the neuropathic pain.
