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Published on: January 30, 2014
[Effects of fractalkine on the expression of inflammatory substances in LPS-activated microglia cells]
Xian-jin Zhu1, Yan-fang Song, Qiu-yu Zhang
1Department of Laboratory, The Affiliated Union Hospital, Fujian Medical University, Fujian Medical University, Fuzhou 350004, China. zxj5027667@tom.com
Aim:
To investigate the effect of fractalkine on the expression of the tumor necrosis factor-α(TNF-α), interleukin-1β (IL-1β), and nitric oxide (NO) in LPS-activated murine microglia cells line N9.
Methods:
In vitro LPS-activated microglia cells were treated for 24 h in the presence of fractalkine. The level of TNF-α and IL-1β in the culture supernatants were measured by enzyme-linked immunosorbent assay (ELISA), The level of NO in the culture supernatants were quantitated by the NO test assay.
Results:
The concentration of TNF-α, IL-1β and NO in the culture supernatants evidently increased in LPS-activated microglia cells groups and prominently decreased by the fractalkine co-incubated.
Conclusion:
It is thus concluded that fractalkine has neuroprotective functions by inhibiting the expression of inflammatory factor in activated microglia cells.
