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Updated: May 26, 2026
Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
Published on: September 9, 2011
[Change in the pattern of peripheral blood TCR V β gene repertoire in occupational lead-exposed workers]
Si-chu Liu1, Bo Li, Yu-zhe Niu
1Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
Aim:
To investigate the distribution and clonality of TCR Vβ subfamily in peripheral blood from workers exposed to lead, in order to understand the change in T cell immunity of occupational lead-exposed workers.
Methods:
The CDR3 of TCR Vβ 24 subfamily genes was amplified in peripheral blood mononuclear cells (PBMC) from 6 lead-exposed workers and 6 healthy individuals using RT-PCR, the positive PCR products were further subjected by genescan analysis to identify T cell clonality.
Results:
All 24 TCR Vβ subfamilies were detected in PBMC from 6 healthy individuals which all have polyclonal patterns. Only 1-7 Vβ subfamilies could be identified in lead-exposed workers. In detected TCR Vβ subfamilies, almost oligoclonal and biclonal patterns which mainly in Vβ1 and Vβ16.
Conclusion:
The restricted expression and clonal expansion of TCR Vβ subfamily have been found in occupational lead-exposed workers, it may have some relationship with lead toxicity damage to the immune function.
