[Mouse anti-human CXCR3 mAb preparation and its biological function]

Area of Science:

• Immunology
• Oncology
• Molecular Biology

Context:

• CXCR3 is a chemokine receptor implicated in immune cell trafficking and cancer progression.
• Understanding CXCR3's role is crucial for developing targeted cancer therapies.

Purpose:

• To generate a hybridoma cell line secreting a stable mouse anti-human CXCR3 monoclonal antibody (mAb).
• To investigate the expression characteristics and functional impact of human CXCR3 on cancer cell migration and proliferation.

Summary:

• A hybridoma cell line (9B5) was successfully established, producing a mouse anti-human CXCR3 mAb (IgG1 subclass).
• The mAb 9B5 effectively binds to CXCR3 on activated T lymphocytes and various colon carcinoma cell lines (Colo205, HCT116, HT29).
• mAb 9B5 demonstrated significant inhibition of cell migration and proliferation, including blocking IP-10-mediated growth promotion in Colo205 cells.

Impact:

• Provides a valuable tool for studying CXCR3's function in tumor growth and metastasis.
• Lays the groundwork for developing novel therapeutic strategies targeting CXCR3 for cancer treatment.
• Offers a potential new avenue for combating tumor metastasis.