Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 26, 2026

A Pilot Study on the Repetitive Transcranial Magnetic Stimulation of Aβ and Tau Levels in Rhesus Monkey Cerebrospinal Fluid
06:22

A Pilot Study on the Repetitive Transcranial Magnetic Stimulation of Aβ and Tau Levels in Rhesus Monkey Cerebrospinal Fluid

Published on: September 3, 2021

[Multicenter study on lumbar puncture indication, clinical practice and feasibility].

C Paquet1, F Latour, I Saulnier

  • 1CMRR Paris Nord Île-de-France, groupe hospitalier Lariboisière Fernand-Widal Saint-Louis, AP-HP, université Denis-Diderot, 2 rue Ambroise-Paré, Paris, France. claire.paquet@inserm.fr

Revue Neurologique
|December 14, 2011
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Progress in the Treatment of Alzheimer's Disease Is Needed - Position Statement of European Alzheimer's Disease Consortium (EADC) Investigators.

The journal of prevention of Alzheimer's disease·2024
Same author

The pattern of cortical thickness associated with executive dysfunction in MCI and SCC: The MEMENTO cohort.

Revue neurologique·2024
Same author

[Translation into French and republication of: "Management of cancer-associated thromboembolism in vulnerable population"].

La Revue de medecine interne·2024
Same author

The free plasma amyloid Aβ<sub>1</sub><sub>-</sub><sub>42</sub>/Aβ<sub>1</sub><sub>-</sub><sub>40</sub> ratio predicts conversion to dementia for subjects with mild cognitive impairment with performance equivalent to that of the total plasma Aβ<sub>1</sub><sub>-</sub><sub>42</sub>/Aβ<sub>1</sub><sub>-</sub><sub>40</sub> ratio. The BALTAZAR study.

Neurobiology of disease·2024
Same author

Orthostatic hypotension: Review and expert position statement.

Revue neurologique·2023
Same author

Orthostatic hypotension and orthostatic hypertension are both associated with lower cognitive function: The S.AGES cohort.

Journal of the American Geriatrics Society·2023

This study examined how lumbar puncture (LP) procedures and cerebrospinal fluid (CSF) handling vary across French Memory Centers. Researchers surveyed 18 centers and found that while most followed guidelines for CSF transport, practices like sample conditioning and premedication varied. A prospective study of 100 patients showed high LP acceptability and safety, with only three minor complications. The authors suggest that these procedural differences may affect diagnostic biomarker results like Aβ and tau proteins. They propose comparing these values across centers to better understand the impact of standardization on Alzheimer's disease diagnostics.

Area of Science:

  • Neurological diagnostics
  • Clinical procedural standardization
  • Alzheimer's disease research

Background:

CSF biomarkers for Alzheimer's disease show variability across studies. Differences in lumbar puncture procedures and CSF handling may contribute to this inconsistency. Prior research has shown that procedural variations can affect biomarker measurements. However, no prior work had resolved how specific steps influence diagnostic accuracy. This gap motivated a multicenter study to assess LP practices and outcomes. Existing knowledge includes the role of Aβ and tau proteins in AD diagnosis. Yet, the impact of standardized LP protocols remains unclear. This paper's contribution is to evaluate current practices and their adherence to guidelines. The study also examines patient tolerance and complication rates.

Purpose Of The Study:

The study aimed to compare LP procedures and CSF transport methods across French Memory Centers. A second goal was to assess LP feasibility and side effects in patients with cognitive issues. Researchers sought to identify variations in clinical practices. They also wanted to evaluate compliance with Innogenetics' recommendations. The study focused on procedural consistency and patient safety. No prior work had resolved how these factors affect biomarker reliability. The researchers proposed that standardization could reduce variability in diagnostic results. This approach addresses a critical need in AD diagnostics.

Keywords:
CSF biomarker variabilitylumbar puncture standardizationAlzheimer's diagnosticsmemory center protocols

Frequently Asked Questions

The study found high acceptability (93/100 patients) and feasibility (97%) of lumbar puncture procedures, with three minor complications reported.

Centers varied in CSF volume, polypropylene tube types, premedication use, and sample conditioning, despite following Innogenetics' guidelines for transport.

The authors propose that inconsistent procedures may affect biomarker reliability, such as Aβ and phosphorylated tau measurements.

Radiological scopes were used successfully for failed lumbar punctures, achieving a 97% overall feasibility rate.

More Related Videos

Spinal Sonography for Ultrasound-Guided Lumbar Neuraxial Anesthesia
03:14

Spinal Sonography for Ultrasound-Guided Lumbar Neuraxial Anesthesia

Published on: January 31, 2025

Related Experiment Videos

Last Updated: May 26, 2026

A Pilot Study on the Repetitive Transcranial Magnetic Stimulation of A&#946; and Tau Levels in Rhesus Monkey Cerebrospinal Fluid
06:22

A Pilot Study on the Repetitive Transcranial Magnetic Stimulation of Aβ and Tau Levels in Rhesus Monkey Cerebrospinal Fluid

Published on: September 3, 2021

Spinal Sonography for Ultrasound-Guided Lumbar Neuraxial Anesthesia
03:14

Spinal Sonography for Ultrasound-Guided Lumbar Neuraxial Anesthesia

Published on: January 31, 2025

Main Methods:

A questionnaire was distributed to all French Memory Centers in May 2010. The survey collected data on LP procedures and CSF transport practices. A prospective study followed 100 patients with cognitive disturbances. Data collection occurred over three weeks and concluded by July 2010. Responses were received from 18 out of 26 centers. The study evaluated LP acceptability, feasibility, and complications. Radiological scopes were used for failed LP attempts. Patient outcomes were tracked for side effects and procedural success.

Main Results:

Eighteen centers responded, revealing varied LP procedures and CSF transport methods. Most followed Innogenetics' guidelines for tubes and transport duration. However, sample conditioning practices differed between centers. Polypropylene tubes varied in type and handling. CSF volume and premedication use were inconsistent. The study found 93 out of 100 patients accepted LP procedures. Feasibility reached 97% with successful follow-up using imaging. Three minor complications were reported. These findings suggest high procedural safety and patient tolerance.

Conclusions:

The study confirmed that French Memory Centers generally follow Innogenetics' pretechnical guidelines. However, each center implemented unique procedures, leading to variability. The authors propose that these differences may affect CSF biomarker measurements. They suggest comparing Aβ, tau, and phosphorylated tau values across centers. The study supports the findings of Zetterberg et al., 2010. High acceptability and safety were observed in the prospective study. The authors emphasize the need for standardized protocols to improve diagnostic consistency. Future work should explore how procedural variations impact biomarker reliability.

The study evaluated 100 patients with cognitive disturbances over a three-week period.

The authors propose that differences in procedures may lead to variability in Aβ, tau, and phosphorylated tau measurements between centers.