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Published on: October 28, 2021
MAPK7 and MAP2K4 as prognostic markers in osteosarcoma.
Francine Tesser-Gamba1, Antonio Sergio Petrilli, Maria Teresa de Seixas Alves
1Department of Pediatrics, Pediatric Oncology Institute (Grupo de Apoio ao Adolescente e à Criança com Câncer), Federal University of São Paulo, São Paulo, SP 04023-062, Brazil. francine.tesser@yahoo.com.br
Osteosarcoma gene expression, particularly MAPK7 and MAP2K4, is linked to poor treatment response and survival. These genes may serve as biomarkers for osteosarcoma progression and treatment effectiveness.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Osteosarcoma, a bone cancer primarily affecting young individuals, is characterized by chromosomal abnormalities.
- Specific chromosomal regions (1p36, 17p, 19) are frequently altered in osteosarcoma, suggesting key gene involvement in its development.
Purpose of the Study:
- To investigate the expression profiles of 10 genes located in chromosomal regions associated with osteosarcoma abnormalities.
- To determine the correlation between the expression of these genes and clinical parameters of osteosarcoma.
Main Methods:
- Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to analyze gene expression.
- Expression levels of 10 genes (MTHFR, ERRFI1, FGR, E2F2, MAPK7, MAP2K4, BBC3, FOSB, JUND, RRAS) were assessed in patient samples and control tissues.
Main Results:
- Overexpression of MAPK7 and MAP2K4 genes showed a significant association with poor response to chemotherapy (P = .0001 and P = .0049).
- Elevated MAPK7 and MAP2K4 expression correlated with tumor progression and reduced overall survival (P = .0052 and P = .0085).
Conclusions:
- MAPK7 and MAP2K4 play a crucial role in osteosarcoma tumorigenesis.
- These genes show potential as biomarkers for predicting treatment response and monitoring osteosarcoma development.
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