Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetic landscape, key transcriptional regulators, and in vivo identification of human Tr1 cells.

Science advances·2026
Same author

XIST RNA-protein complex in female-biased autoimmunity: From molecular scaffolds to new clinical biomarkers.

The Journal of investigative dermatology·2026
Same author

Somatic mutations reveal the ontogeny of human microglia.

bioRxiv : the preprint server for biology·2026
Same author

Correction: Annotation of nuclear lncRNAs based on chromatin interactions.

PloS one·2026
Same author

Fast and accurate resolution of ecDNA sequence using Cycle-Extractor.

bioRxiv : the preprint server for biology·2026
Same author

A recipe for chaos: Extrachromosomal DNA and the hallmarks of cancer.

Cell·2026

Related Experiment Video

Updated: May 26, 2026

RNA-Associated Chromatin DNA-DNA Interaction Method
11:01

RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

Active chromatin and noncoding RNAs: an intimate relationship.

Ryan A Flynn1, Howard Y Chang

  • 1Howard Hughes Medical Institute and Program in Epithelial Biology, Stanford University School of Medicine, Stanford, CA 94305, United States.

Current Opinion in Genetics & Development
|December 14, 2011
PubMed
Summary
This summary is machine-generated.

Long noncoding RNAs (lncRNAs) are newly found to regulate active chromatin states. These noncoding RNAs influence gene expression by altering histone methylation, establishing active chromatin domains.

More Related Videos

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

Chromatin Isolation by RNA Purification (ChIRP)
11:09

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

Related Experiment Videos

Last Updated: May 26, 2026

RNA-Associated Chromatin DNA-DNA Interaction Method
11:01

RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

Chromatin Isolation by RNA Purification (ChIRP)
11:09

Chromatin Isolation by RNA Purification (ChIRP)

Published on: March 25, 2012

Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Eukaryotic genomes are organized into chromatin, with histone modifications defining gene-regulatory domains.
  • Long noncoding RNAs (lncRNAs) have been linked to silent chromatin, but their role in active states is emerging.
  • Noncoding RNAs can influence gene expression by modulating chromatin states.

Purpose of the Study:

  • To investigate the role of noncoding RNAs in modulating active chromatin states.
  • To explore how different types of noncoding RNAs affect gene expression.
  • To understand the mechanisms by which noncoding RNAs establish active chromatin domains.

Main Methods:

  • Analysis of diverse noncoding RNA types (divergent, antisense, enhancer-like).
  • Investigation of effects on histone H3 lysine 4 methylation.
  • Exploration of the link between chromosome structure and chromatin state.

Main Results:

  • Noncoding RNAs, including enhancer-like lncRNAs, can modulate active chromatin.
  • These RNAs influence gene expression by altering histone H3 lysine 4 methylation.
  • Emerging evidence suggests lncRNAs link chromosome structure to active chromatin domains.

Conclusions:

  • Noncoding RNAs play a significant role in regulating active chromatin.
  • Histone methylation is a key mechanism through which noncoding RNAs exert their effects.
  • Future technologies will accelerate research into noncoding RNA functions in epigenetics.