Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 26, 2026

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform
09:35

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

Published on: July 16, 2016

A human islet cell culture system for high-throughput screening.

Deepika Walpita1, Thomas Hasaka, James Spoonamore

  • 1Chemical Biology Program, Broad Institute, Cambridge, MA 02142, USA.

Journal of Biomolecular Screening
|December 14, 2011
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Harmine Selectively Drives Human Beta Cell Differentiation and Function Via Protein Kinase A Pathways.

bioRxiv : the preprint server for biology·2026
Same author

Small molecule splicing modulators that disrupt O-GlcNAc homeostasis.

Nature communications·2026
Same author

Author Correction: Diversity-oriented synthesis yields novel multistage antimalarial inhibitors.

Nature·2025
Same author

Human pancreatic α-cell heterogeneity and trajectory inference analyses reveal SMOC1 as a β-cell dedifferentiation gene.

Nature communications·2025
Same author

SIRT2 puts the brakes on human β cell proliferation: therapeutic opportunities and next challenges.

The Journal of clinical investigation·2025
Same author

Multiomics Analysis Reveals Insights into Potential Drivers of Pancreatic Islet Pathology in Type 2 Diabetes.

ACS omega·2025

Researchers developed a high-throughput screening method using human islets to find small molecules that promote beta-cell proliferation, a key step for type 1 diabetes regeneration therapies.

Area of Science:

  • Endocrinology
  • Cell Biology
  • Regenerative Medicine

Background:

  • Type 1 diabetes treatment strategies focus on beta-cell regeneration.
  • Developing effective human beta-cell lines for research is challenging.
  • A high-throughput screening method is needed to identify beta-cell proliferation inducers.

Purpose of the Study:

  • To adapt an islet cell culture system for high-throughput screening.
  • To identify small molecules that induce human beta-cell proliferation.
  • To assess beta-cell function in a novel culture environment.

Main Methods:

  • Human islets were cultured on extracellular matrix from a human bladder carcinoma cell line in microtiter plates.
  • Beta-cell proliferation was assessed using Ki67 and C-peptide immunofluorescence.

More Related Videos

Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles
08:04

Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles

Published on: November 3, 2023

Related Experiment Videos

Last Updated: May 26, 2026

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform
09:35

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

Published on: July 16, 2016

Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles
08:04

Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles

Published on: November 3, 2023

  • Glucose-stimulated insulin secretion was measured to confirm physiological function.

    • Main Results:

    • The culture system preserved beta-cell physiological function.
    • Adenoviral induction of cdk-6 and cyclin D1 promoted proliferation, which was inhibited by rapamycin.
    • A pilot screen of 1280 compounds revealed phenotypic effects.

    Conclusions:

    • A high-throughput human islet cell culture method was established.
    • This method enables the assessment of beta-cell biology and compound screening.
    • This approach holds potential for discovering type 1 diabetes therapeutics.