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Updated: May 26, 2026
Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
Published on: October 21, 2022
Measurements of microkeratome cuts in donor corneas with ultrasound and optical coherence tomography
Maolong Tang1, Donald Ward, Jose Luiz Branco Ramos
1Center for Ophthalmic Optics and Lasers, Doheny Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Purpose:
To evaluate optical coherence tomography (OCT) in the measurement of donor corneas in preparation for endothelial keratoplasty.
Methods:
Donor corneas were imaged by OCT while immersed in preservation medium. Central corneal thickness (CCT) was measured by OCT from the Bowman layer to the endothelium. The corneas were then mounted on an artificial anterior chamber, and the epithelium was removed. Ultrasound pachymetry (USP) was used to measure CCT just before sectioning with a microkeratome. The central graft thickness (CGT) was measured by USP. The graft was then returned to the medium and imaged by OCT.
Results:
The study included 154 donor corneas. The average CCT measured by OCT (550 ± 63 microm) was thicker (P < 0.001) than that measured by USP (507 ± 54 microm). Similarly, the CGT measured by OCT (158 ± 41 microm) was thicker (P = 0.0005) than that measured by USP (153 ± 38 microm). The predictability of cut depth, as assessed by pooled standard deviation (SD), was better (P = 0.023) for USP (41 microm) compared with OCT (48 microm). The graft was thicker (P < 0.001) peripherally than centrally in OCT images. The predictability of cut depth by OCT was better (P < 0.001) for corneas thinner than 600 microm (SD = 45.6 microm) compared with those thicker than 600 microm (SD = 86.9 microm).
Conclusions:
The donor corneal measurements by OCT were not as predictable as those by USP. The predictability of graft thickness, however, could be optimized using OCT to select for corneas thinner than 600 microm and then using immediate precut USP to set the microkeratome depth. A graft thickness profile measured by OCT could be useful to the surgeon.
