Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Testes: Histology

Testes: Histology

A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
The spermatogenic cells, responsible for producing sperm, are...

Testes: Gross Anatomy

Testes: Gross Anatomy

The testes, also known as testicles, are the male gonads. They are housed within the scrotum, a sac-like structure located beneath the penis. The scrotum's primary role is to regulate the temperature of the testes, which is crucial for sperm production.
Each testis is surrounded by the tunica albuginea, a dense connective tissue layer that provides structural support and protection. This layer is covered by an outer serous membrane called the tunica vaginalis, which helps reduce friction...

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...

Spermatogenesis

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...

Development of the Sexual Organs in the Embryo and Fetus

Development of the Sexual Organs in the Embryo and Fetus

Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the male...

Sperm Transport

Sperm Transport

The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Immunoaffinity-Based Protocol to Enrich Nervous System Cell-, Lung Alveolar Cell-, and Hepatocyte-Derived Extracellular Vesicles From Human Plasma.

Journal of extracellular biology·2026

Same author

Advancements in extracellular vesicle research.

Extracellular vesicle·2026

Same author

A high-throughput, end-to-end pipeline for extracellular miRNA biomarker discovery from human biofluids.

Cell reports methods·2026

Same author

Fetal monitoring for high-risk pregnancies using a wearable ultrasound patch.

Nature biotechnology·2026

Same author

Cost-Effectiveness of Recombinant Zoster Vaccine (Shingrix) in US Adults Aged ≥50 Years.

The American journal of medicine·2026

Same author

Development and Validation of Flow Cytometry-Based Method to Quantify CD34/CD45+ Cells as a Release Criterion of Clinical-Grade Products for a Phase III Clinical Trial.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026

Search research articles

Related Experiment Video

Updated: May 26, 2026

Teratoma Generation in the Testis Capsule

Teratoma Generation in the Testis Capsule

Published on: November 7, 2011

Teratoma generation in the testis capsule.

Suzanne E Peterson¹, Ha T Tran, Ibon Garitaonandia

¹Department of Chemical Physiology, Scripps Research Institute.

Journal of Visualized Experiments : Jove

|December 14, 2011

Summary

Human pluripotent stem cells (hPSCs) can be assessed for pluripotency using the teratoma assay. This study details a robust protocol for generating teratomas from hPSCs in immune-compromised mice, utilizing the testis capsule as the injection site.

More Related Videos

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Published on: August 1, 2010

Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture

Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture

Published on: October 3, 2019

Related Experiment Videos

Last Updated: May 26, 2026

Teratoma Generation in the Testis Capsule

Teratoma Generation in the Testis Capsule

Published on: November 7, 2011

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Published on: August 1, 2010

Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture

Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture

Published on: October 3, 2019

Area of Science:

Stem cell biology
Regenerative medicine

Background:

Pluripotent stem cells (PSCs) can differentiate into all cell types, offering therapeutic potential.
Assessing pluripotency is crucial for human pluripotent stem cells (hPSCs), as germline transmission assays used for mouse ESCs are not applicable.
The teratoma assay is the current standard for evaluating hPSC pluripotency, despite ongoing research into alternative methods.

Purpose of the Study:

To describe a robust protocol for generating teratomas from hPSCs.
To establish the testis capsule as a reliable site for hPSC teratoma formation.

Main Methods:

Human pluripotent stem cells (hPSCs) were injected into the testis capsule of immune-compromised mice.
Teratoma formation and subsequent histological analysis were performed to assess pluripotency.

Main Results:

Teratomas were successfully generated from hPSCs.
Histological examination confirmed the presence of tissues from all three germ layers within the teratomas, indicating pluripotency.

Conclusions:

The described protocol provides a reliable method for teratoma generation from hPSCs.
The testis capsule is an effective site for hPSC teratoma formation, supporting its use in pluripotency assessment.