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Differentiation antigens on normal and Abelson virus transformed lymphocytes
Journal of Immunology (Baltimore, Md. : 1950)
|June 1, 1979
Summary
Researchers identified novel membrane antigens on Abelson leukemia virus-induced lymphomas. These antigens suggest that certain lymphomas may originate from transformed B cell precursors, indicating potential B cell differentiation states.
Area of Science:
- Immunology
- Virology
- Oncology
Background:
- Abelson leukemia virus (A-MuLV) is known to induce murine lymphomas.
- Understanding the cell origins of these lymphomas is crucial for developing targeted therapies.
Purpose of the Study:
- To identify novel cell surface antigens on A-MuLV-induced murine lymphomas.
- To determine the differentiation state and cell lineage of A-MuLV-induced lymphomas.
Main Methods:
- Rabbit antisera against A-MuLV-induced lymphomas were generated.
- Absorption analysis with various murine lymphoma lines was performed.
- Hapten-sandwich indirect membrane immunofluorescence was used to visualize antibody binding.
Main Results:
- Novel membrane antigens were detected.
- These antigens are shared between lymphosarcomas, B lymphomas, normal B lymphocytes, and normal immunoglobulin-negative bone marrow cells.
- Antigens were not found on T cells, thymic lymphomas, plasmacytoid lymphomas, or myelomas.
Conclusions:
- The detected antigens suggest that immunoglobulin-negative A-MuLV-induced lymphosarcomas may arise from transformed B cell precursors.
- Variations in antigen expression indicate that plasmacytoid lymphomas may represent diverse differentiation states.