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Related Experiment Video

Updated: May 26, 2026

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
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Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Published on: September 13, 2022

Addressing diffuse glioma as a systemic brain disease with single-cell analysis.

Felix Sahm1, David Capper, Astrid Jeibmann

  • 1Department of Neuropathology, Institute of Pathology, German Cancer Research Center, Heidelberg, Germany.

Archives of Neurology
|December 14, 2011
PubMed
Summary

Single tumor cells from IDH1 mutant diffuse gliomas were found throughout the brain. This suggests diffuse gliomas are a systemic brain disease, not localized tumors.

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Related Experiment Videos

Last Updated: May 26, 2026

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma
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Published on: September 13, 2022

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09:09

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Published on: April 12, 2020

Area of Science:

  • Neuro-oncology
  • Cancer biology
  • Immunohistochemistry

Background:

  • Diffuse gliomas are primary brain tumors.
  • IDH1 mutations are common in diffuse gliomas.
  • Understanding glioma infiltration is crucial for treatment.

Observation:

  • Used immunohistochemistry with an IDH1 R132H specific antibody.
  • Analyzed whole-brain and hemisphere sections from 4 glioma patients.
  • Identified single tumor cells in macroscopically and histologically inconspicuous areas.

Findings:

  • Single tumor cells were detected throughout the brain in all patients.
  • Tumor cells infiltrated areas without apparent abnormalities.
  • This infiltration occurred irrespective of cellularity, pleomorphism, or mitotic activity.

Implications:

  • Diffuse gliomas exhibit systemic spread within the brain.
  • Current treatment strategies may need to account for widespread infiltration.
  • This finding redefines diffuse gliomas as a systemic brain disease.