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Related Experiment Video

Updated: May 26, 2026

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

Published on: April 16, 2012

Prolactin down-regulates CD4+CD25hiCD127low/- regulatory T cell function in humans.

M V Legorreta-Haquet¹, K Chávez-Rueda, E Montoya-Díaz

¹Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, CMN Siglo XXI, Mexico, DF, Mexico.

Journal of Molecular Endocrinology

|December 14, 2011

Summary

Prolactin (PRL) regulates immune responses. This study found that prolactin inhibits regulatory T (Treg) cell function and promotes Th1 cytokine secretion, impacting T cell activation and differentiation.

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Area of Science:

Immunology
Endocrinology
Cell Biology

Background:

Prolactin (PRL) is known to influence immune responses, including T cell activation, differentiation, and proliferation.
The specific mechanisms by which PRL regulates regulatory T (Treg) cells remain largely unelucidated.
Understanding PRL's role in Treg cell function is crucial for comprehending immune system regulation.

Purpose of the Study:

To investigate the role of prolactin (PRL) in the function of regulatory T (Treg) cells.
To determine the expression patterns of PRL and its receptor in Treg and effector T (Teff) cells.
To assess the impact of PRL on Treg cell-mediated suppression and cytokine secretion.

Main Methods:

Quantified PRL and PRL receptor expression in human Treg and Teff cells.
Assessed Treg cell functional activity, including proliferation and cytokine secretion, in response to anti-CD3/CD28 stimulation with and without PRL.
Utilized co-culture systems to evaluate the suppressive capacity of Treg cells in the presence of PRL.

Main Results:

Treg cells constitutively express the PRL receptor, while Teff cells require stimulation to induce its expression.
Prolactin significantly inhibited the suppressive function of Treg cells in vitro, reducing proliferation.
PRL treatment promoted a Th1 cytokine profile, increasing the production of TNF and IFNγ.

Conclusions:

PRL receptor expression is constitutive in Treg cells but inducible in Teff cells.
Prolactin actively inhibits the suppressive function of Treg cells.
PRL-induced inhibition of Treg cells may occur via the promotion of Th1 cytokine secretion.