Related Experiment Video
Updated: May 26, 2026
Half-segmental Diaphyseal Bone Defect Model in Rats for Evaluating Bone Substitute Performance in Load-bearing Regions
Published on: December 30, 2025
Castor oil polyurethane containing silica nanoparticles as filling material of bone defect in rats
Renato Silva Nacer1, Rodrigo Ré Poppi, Paulo de Tarso Camilo de Carvalho
1Health and Development in the West Central Region, Federal University of Mato Grosso do Sul (UFMS), Campo Grande-MS, Brazil. renatosnacer@gmail.com
Purpose:
To evaluate the biologic behavior of the castor polymer containing silica nanoparticles as a bone substitute in diafisary defect.
Methods:
Twenty seven male Rattus norvegicus albinus Wistar lineage were submitted to bone defect filled with castor oil polymer. Three experimental groups had been formed with nine animals each: (1) castor oil polymer containing only calcium carbonate; (2) castor oil polymer with calcium carbonate and doped with 5% of silica nanoparticles; (3) castor polymer with calcium carbonate doped with 10% of silica nanoparticles; 3 animals of each group were submitted to euthanasia 15, 30 and 60 days after experimental procedure, and their femurs were removed to histological evaluation.
Results:
there was bone growth in all the studied groups, with a greater tendency of growth in the group 1. After 30 days all the groups presented similar results. After 60 days a greater amount of fibroblasts, osteoblasts, osteocytes and osteoclasts in group 3 was observed, with integrated activity of 3 kinds of cells involved in the bone activation-reabsorption-formation.
Conclusions:
The castor polymer associated to the silica nanoparticles is biocompatible and allows osteoconduction. The presence of osteoprogenitors cells suggests silica osteoinduction capacity.
