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Related Experiment Video

Updated: May 26, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Array-based identification of common DNA methylation alterations in ulcerative colitis.

Kei Koizumi1, Sergio Alonso, Yuichiro Miyaki

  • 1First Department of Surgery, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.

International Journal of Oncology
|December 14, 2011
PubMed
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Long-standing ulcerative colitis (UC) patients show altered DNA methylation patterns, potentially predicting colorectal cancer risk. This study reveals the first high-throughput epigenetic profiles in UC colonic mucosa.

Area of Science:

  • Genomics
  • Epigenetics
  • Gastroenterology

Background:

  • Long-standing ulcerative colitis (UC) increases colorectal cancer (CRC) risk.
  • Epigenetic alterations are implicated in UC-associated carcinogenesis.
  • Understanding these molecular changes is crucial for risk prediction.

Purpose of the Study:

  • To develop a high-throughput platform for analyzing genome-wide DNA methylation in UC.
  • To identify aberrant DNA methylation profiles in the colonic mucosa of UC patients.
  • To explore the potential of these epigenetic alterations as CRC risk predictors.

Main Methods:

  • Developed a novel microarray platform using methylation-sensitive amplified fragment length polymorphism (MS-AFLP) DNA fingerprinting.
  • Utilized synthetic primers targeting over 10,000 NotI sites across the genome.

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Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
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Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

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Last Updated: May 26, 2026

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13:47

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  • Investigated DNA methylation in colonic mucosa from 14 UC patients and a colon cancer cell line (CW-2).

    • Main Results:

    • The MS-AFLP platform demonstrated reproducibility and sensitivity.
    • Identified significant DNA methylation alterations (hyper- and hypomethylation) in UC colonic mucosa.
    • Observed concordance in methylation patterns between UC samples and colon cancer cells, suggesting a field defect.

    Conclusions:

    • This study presents the first high-throughput aberrant DNA methylation profiles for UC colonic mucosa.
    • These epigenetic profiles offer insights into molecular alterations in UC pathology.
    • Detected methylation alterations may serve as potential biomarkers for CRC risk prediction in UC patients.