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Related Experiment Video

Updated: May 26, 2026

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
08:27

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Published on: April 16, 2014

A digital microfluidic method for multiplexed cell-based apoptosis assays.

Dario Bogojevic1, M Dean Chamberlain, Irena Barbulovic-Nad

  • 1Institute for Biomaterials and Biomedical Engineering, University of Toronto, 164 College St., Toronto, ON M5S 3G9, Canada.

Lab on a Chip
|December 14, 2011
PubMed
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This summary is machine-generated.

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Digital microfluidics (DMF) enables parallel cell-based assays with reduced reagent use. This new platform offers lower detection limits and greater dynamic range for drug discovery screening.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell-based assays

Background:

  • Digital microfluidics (DMF) manipulates small liquid volumes electrostatically.
  • DMF is reconfigurable, has no moving parts, and integrates with high-throughput screening.
  • DMF shows promise for multiplexed screening applications in chemistry and biology.

Purpose of the Study:

  • Introduce the first digital microfluidic platform for parallel-scale cell-based assays.
  • Utilize a fluorogenic apoptosis assay for caspase-3 activity as a model system.
  • Evaluate DMF's utility in anti-cancer drug discovery research.

Main Methods:

  • Developed a novel digital microfluidic platform for cell-based assays.
  • Implemented a fluorogenic apoptosis assay targeting caspase-3 activity.

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  • Compared DMF assay results with conventional pipetting/aspiration in 96-well plates.

    • Main Results:

    • Achieved a 33-fold reduction in reagent consumption using DMF compared to conventional methods.
    • DMF assays exhibited lower detection limits and a greater dynamic range.
    • Minimized cell de-lamination during droplet manipulation with DMF.

    Conclusions:

    • The described DMF platform represents a significant advancement for parallel cell-based screening.
    • DMF offers advantages in reagent efficiency, sensitivity, and dynamic range for biological assays.
    • This technology is a valuable tool for anti-cancer drug discovery and other applications.