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Updated: May 26, 2026
DNA Vector-based RNA Interference to Study Gene Function in Cancer
Published on: June 4, 2012
Lentivirus-mediated siRNA interference targeting SGO-1 inhibits human NSCLC cell growth
Lili Liu1, Ning Zhang, Jiayu Liu
1Department of Oncology, Tangdu Hospital, Fourth Military Medical University, 1 Xinsi Road, Xi'an, 710038, China.
Abstract:
Aneuploid is recognized as a hallmark of cancer and is caused by chromosome mis-segregation during mitosis. Recent studies have identified shugoshin (SGO-1) as an important player in proper chromosome segregation and the involvement in tumorigenesis. But, little is known about how SGO-1 is involved. The aim of this study was to obtain information about the role of SGO-1 in human lung cancer cells. In our study, real-time PCR and western blotting assay were performed to detect the expression of SGO-1 in nonsmall cell lung cancer (NSCLC) cell lines. Then, we successfully constructed a lentivirus vector mediating RNAi targeting of SGO-1 (LV-SGO-1 siRNA) and proved that it can suppress the expression of SGO-1 gene in H1299 cells. We found that SGO-1 was highly expressed in NSCLC cancer cell lines. RNA interference of SGO-1 by the LV-SGO-1 siRNA construct significantly decreased SGO-1 protein expression and inhibited the growth and ability of forming colonies of H1299 cells with more cells arrested in G2/M phase, but lentivirus vector control had no effect on H1299 cells. Moreover, suppression of SGO-1 by LV-SGO-1 siRNA increased the apoptosis of H1299 cells with down-regulated Bcl-2 and up-regulated Bax. SGO-1 may provide a novel therapeutic target for the treatment of lung cancer since it inhibits the growth and increases the apoptosis of lung cancer cells.
Insights
Shugoshin (SGO-1) is highly expressed in non-small cell lung cancer (NSCLC) and its suppression inhibits cancer cell growth and promotes apoptosis. Targeting SGO-1 may offer a new therapeutic strategy for lung cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Aneuploidy, a hallmark of cancer, arises from chromosome mis-segregation during mitosis.
- Shugoshin (SGO-1) is implicated in chromosome segregation and tumorigenesis, but its precise role in lung cancer is unclear.
Purpose of the Study:
- To investigate the role of shugoshin (SGO-1) in human lung cancer cells.
- To determine if SGO-1 is a potential therapeutic target for non-small cell lung cancer (NSCLC).
Main Methods:
- Real-time PCR and western blotting to assess SGO-1 expression in NSCLC cell lines.
- Construction and application of a lentivirus vector for SGO-1 RNA interference (LV-SGO-1 siRNA).
- Assessment of cell proliferation, colony formation, cell cycle, and apoptosis in H1299 cells following SGO-1 suppression.
Main Results:
- SGO-1 was found to be highly expressed in NSCLC cell lines.
- RNA interference targeting SGO-1 significantly reduced its protein expression in H1299 cells.
- Suppression of SGO-1 inhibited cell growth, colony formation, and induced G2/M phase arrest, while increasing apoptosis via modulation of Bcl-2 and Bax expression.
Conclusions:
- SGO-1 plays a crucial role in the proliferation and survival of lung cancer cells.
- Down-regulation of SGO-1 inhibits tumor growth and induces apoptosis in lung cancer cells.
- SGO-1 represents a promising novel therapeutic target for lung cancer treatment.
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