Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Redox Reactions01:24

Redox Reactions

Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dissecting mechanisms of ligand binding and conformational changes in the glutamine-binding protein.

eLife·2026
Same author

Allosteric Regulation of Photophysics and Binding in Oxazine-macrocycle Complexes at Single-molecule Resolution.

Angewandte Chemie (International ed. in English)·2026
Same author

A Systematic Approach for the Purification of Fluorophore-Labeled Proteins via Anion Exchange Chromatography.

Chembiochem : a European journal of chemical biology·2026
Same author

Modulation of Single-Molecule Emission at Hexagonal Boron Nitride Surfaces.

Nano letters·2026
Same author

Single-Molecule Forster Resonance Energy Transfer With a Minimalistic 3D-Printed Setup and Dyes in the Blue-Green Spectral Region.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Detergent Conjugation Decouples Chlorpromazine Solubility from Antibacterial Activity against Bacteria Relevant for Gut Microbiota.

Bioconjugate chemistry·2026

Related Experiment Video

Updated: May 26, 2026

Observation and Analysis of Blinking Surface-enhanced Raman Scattering
05:52

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Published on: January 11, 2018

Linking single-molecule blinking to chromophore structure and redox potentials.

Ingo H Stein1, Stella Capone, Jochem H Smit

  • 1NanoBioScience Group, Institute for Physical and Theoretical Chemistry, TU Braunschweig, Hans-Sommer-Str. 10, 38106 Braunschweig, Germany.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|December 14, 2011
PubMed
Summary

Blinking in luminescent dyes like Cy3, Cy5, and Cy7 shows longer OFF-times with increasing dye size. This is linked to their reduction potentials, offering insights into dark states in microscopy.

More Related Videos

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
11:22

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Related Experiment Videos

Last Updated: May 26, 2026

Observation and Analysis of Blinking Surface-enhanced Raman Scattering
05:52

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

Published on: January 11, 2018

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
11:22

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Area of Science:

  • Photophysics and Spectroscopy
  • Chemical Biology
  • Materials Science

Background:

  • Luminescent molecules exhibit blinking (ON/OFF-state fluctuations) at the single-molecule level.
  • Understanding blinking dynamics is crucial for applications like super-resolution microscopy.

Purpose of the Study:

  • To investigate the blinking behavior of cyanine dyes (Cy3, Cy5, Cy7) under varying oxidant conditions.
  • To correlate OFF-state lifetimes with the redox potentials of the dyes.

Main Methods:

  • Studied blinking of Cy3, Cy5, and Cy7 dyes.
  • Induced radical anion states by controlling oxidant (oxygen) and reductant (ascorbic acid) levels.
  • Analyzed reaction rates and compared with Rehm-Weller plots using methylviologen as an oxidant.

Main Results:

  • OFF-state lifetime increased in the order Cy3 < Cy5 < Cy7.
  • Longer OFF-times correlated with higher reduction potentials, increasing with chromophore size.
  • Observed unexpectedly low radical anion reaction rates at typical oxygen concentrations.

Conclusions:

  • OFF-state lifetimes are influenced by fluorophore reduction potentials and chromophore size.
  • The findings support a model of photoinduced reduction and oxidation reactions.
  • Relating OFF-state lifetimes to redox potentials may help predict dark state behavior in microscopy.