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Related Experiment Video

Updated: May 26, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Blood testing at the single cell level using quantitative phase and amplitude microscopy.

Mustafa Mir, Krishnarao Tangella, Gabriel Popescu

    Biomedical Optics Express
    |December 14, 2011
    PubMed
    Summary

    Researchers developed a new method combining quantitative phase imaging with absorption measurements to simultaneously determine red blood cell thickness and refractive index. This breakthrough enables more detailed and sensitive red blood cell analysis for clinical applications.

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    Area of Science:

    • Biomedical Optics
    • Cellular Imaging
    • Hematology

    Background:

    • Quantitative phase imaging (QPI) offers detailed red blood cell (RBC) analysis.
    • Simultaneous measurement of RBC thickness and refractive index is crucial for clinical translation but challenging.
    • Current QPI methods face limitations in practical, simultaneous thickness and refractive index determination.

    Purpose of the Study:

    • To develop and validate a method for simultaneously measuring RBC thickness and refractive index.
    • To overcome the limitations of current QPI techniques for clinical RBC analysis.
    • To enable precise, single-cell level characterization of RBC properties.

    Main Methods:

    • Combined quantitative phase imaging with a single absorption measurement.
    Keywords:
    (170.0180) Microscopy(170.1470) Blood or tissue constituent monitoring(170.1530) Cell analysis(170.1610) Clinical applications(180.3170) Interference microscopy(300.1030) Absorption

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    Last Updated: May 26, 2026

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
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    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

    Published on: April 7, 2014

    Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
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  • Developed a theoretical framework and conducted experimental validation.
  • Compared results with a clinical blood analyzer for accuracy.

    • Main Results:

    • Successfully demonstrated simultaneous measurement of RBC thickness and refractive index at the single-cell level.
    • Validated the accuracy of the combined imaging approach against clinical standards.
    • The method effectively decouples thickness and refractive index for RBCs.

    Conclusions:

    • The combined quantitative phase and absorption imaging method provides a practical approach for simultaneous RBC thickness and refractive index measurement.
    • This technique enhances the potential for clinical translation of QPI in hematology.
    • Applicable to various QPI methods compatible with bright-field microscopy at the Soret-band wavelength.