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Label-free Single Molecule Detection Using Microtoroid Optical Resonators
08:53

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Published on: December 29, 2015

Bioconjugation strategies for microtoroidal optical resonators.

Heather K Hunt1, Carol Soteropulos, Andrea M Armani

  • 1Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA 90089, USA. heather.hunt@usc.edu

Sensors (Basel, Switzerland)
|December 14, 2011
PubMed
Summary

Researchers developed a new method to make label-free biosensors more specific without harming their sensitivity. This technique uses covalent functionalization for precise detection in medical diagnostics and environmental monitoring.

Keywords:
bioconjugationhigh quality factoroptical resonatorssensors

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Last Updated: May 26, 2026

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
08:53

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Published on: December 29, 2015

Fabrication of Silica Ultra High Quality Factor Microresonators
07:51

Fabrication of Silica Ultra High Quality Factor Microresonators

Published on: July 2, 2012

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
09:35

Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents

Published on: May 1, 2012

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Materials Science

Background:

  • Label-free biosensors are crucial for medical diagnostics and environmental monitoring, requiring high sensitivity and specificity.
  • Optical resonant devices offer high sensitivity but often lack specificity due to challenges in surface functionalization.
  • Previous efforts focused on sensor development, neglecting robust methods for achieving specificity without compromising optical performance.

Purpose of the Study:

  • To develop a facile covalent surface functionalization method for optical microcavities.
  • To impart specificity to microtoroidal resonators without negatively impacting their optical performance.
  • To demonstrate a non-physisorption-based bioconjugation technique for microcavity biosensors.

Main Methods:

  • Selective surface functionalization of silica microtoroids with biotin using amine-terminated silane coupling agents.
  • Characterization of surface chemistry using X-ray photoelectron spectroscopy, fluorescent, and optical microscopy.
  • Assessment of device quality factors to evaluate the impact of functionalization on sensitivity.

Main Results:

  • Demonstrated a facile method for specific surface functionalization of optical microcavities.
  • Confirmed uniform surface coverage and no microstructural damage to the devices.
  • Maintained or minimally impacted the optical performance (quality factors) of the microtoroids.

Conclusions:

  • A robust covalent functionalization strategy was successfully implemented for microtoroidal resonators.
  • This method enhances biosensor specificity while preserving high sensitivity, crucial for real-time detection applications.
  • Represents a significant advancement in bioconjugation techniques for optical biosensing platforms.