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Updated: May 26, 2026

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
Published on: July 26, 2019
Theory and applications of surface plasmon resonance, resonant mirror, resonant waveguide grating, and dual
Hikmat N Daghestani1, Billy W Day
1Department of Structural Biology, University of Pittsburgh, BST3 10017, 3501 Fifth Ave, Pittsburgh, PA 15213, USA. hnd3@pitt.edu
Abstract:
Biosensors have been used extensively in the scientific community for several purposes, most notably to determine association and dissociation kinetics, protein-ligand, protein-protein, or nucleic acid hybridization interactions. A number of different types of biosensors are available in the field, each with real or perceived benefits over the others. This review discusses the basic theory and operational arrangements of four commercially available types of optical biosensors: surface plasmon resonance, resonant mirror, resonance waveguide grating, and dual polarization interferometry. The different applications these techniques offer are discussed from experiments and results reported in recently published literature. Additionally, recent advancements or modifications to the current techniques are also discussed.
