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Related Experiment Video

Updated: May 26, 2026

Thermal Measurement Techniques in Analytical Microfluidic Devices
08:29

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

A high-precision micropipette sensor for cellular-level real-time thermal characterization.

Ramesh Shrestha1, Tae-Youl Choi, Wonseok Chang

  • 1Department of Mechanical and Energy Engineering, University of North Texas, 3940 N. Elm St, Denton, TX 76207, USA. rameshstha@yahoo.com

Sensors (Basel, Switzerland)
|December 14, 2011
PubMed
Summary

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We developed a novel, cost-effective glass micropipette thermal sensor for precise temperature measurements at the micrometer scale. This sensor accurately detects thermal fluctuations in biological cells, advancing cellular thermal analysis.

Area of Science:

  • Biophysics
  • Microscale thermal sensing
  • Cellular thermometry

Background:

  • Accurate measurement of temperature at the microscale is crucial for understanding cellular processes.
  • Existing thermal sensing technologies often lack the required spatial resolution or are prohibitively expensive.

Purpose of the Study:

  • To develop a novel, cost-effective glass micropipette thermal sensor.
  • To achieve high spatial resolution (∼2 μm) and accuracy (±0.01 °C) for thermal measurements.
  • To demonstrate the sensor's capability for cellular-level temperature mapping.

Main Methods:

  • Fabrication of a glass micropipette sensor using borosilicate glass, Sn-based solder alloy, and nickel coating.
  • Calibration of the sensor in a controlled thermal chamber (±0.01 °C accuracy).
Keywords:
cellular-levellasermicropipettethermal sensor

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  • Measurement of temperature changes within live retinal pigment epithelium cells subjected to laser irradiation.

    • Main Results:

    • Successfully fabricated and tested micrometer-sized thermal sensors (2-30 μm).
    • Achieved a Seebeck coefficient of 8.46–8.86 μV/°C.
    • Demonstrated in-situ measurement of transient temperature profiles in cells (38–55 ± 0.5 °C) with a 6 μm laser spot.

    Conclusions:

    • The novel glass micropipette thermal sensor offers a cost-effective solution for high-resolution, accurate temperature measurements.
    • The sensor is capable of probing thermal dynamics at the cellular level.
    • This technology has significant potential for applications in cell biology and biophysics.