Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Biofuels

Biofuels

The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...

Hydroboration-Oxidation of Alkenes

Hydroboration-Oxidation of Alkenes

In addition to the oxymercuration–demercuration method, which converts the alkenes to alcohols with Markovnikov orientation, a complementary hydroboration-oxidation method yields the anti-Markovnikov product. The hydroboration reaction, discovered in 1959 by H.C. Brown, involves the addition of a B–H bond of borane to an alkene giving an organoborane intermediate. The oxidation of this intermediate with basic hydrogen peroxide forms an alcohol.

Bioplastics

Bioplastics

Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...

Fates of Pyruvate

Fates of Pyruvate

Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...

Insulin: Biosynthesis, Chemistry, and Preparation

Insulin: Biosynthesis, Chemistry, and Preparation

The endoplasmic reticulum (ER) of pancreatic β-cells synthesizes preproinsulin, which consists of a signal peptide, A and B chains, and a C-peptide. Preproinsulin is then cleaved and folded into proinsulin, which translocates to the Golgi apparatus for sorting and packaging into secretory granules. In these granules, enzymatic clipping generates insulin and C-peptide.
Damage or functional impairment of β-cells inhibits insulin production, leading to diabetes. Diabetes treatment primarily uses...

Drug Biotransformation: Overview

Drug Biotransformation: Overview

Pharmaceutical substances known as xenobiotics are predominantly lipophilic and nonionized. This enables them to permeate lipid bilayers, such as cell membranes, and interact with intracellular target receptors. Lipophilic drugs have an advantage in crossing biological barriers and reaching their intended sites of action. However, lipophilic drugs often have a restricted capacity for renal expulsion or elimination from the body. When these drugs enter the kidneys and undergo glomerular...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Overall Water-Splitting Enabled by Bifunctional NiPd/Pd Heterodimer Fabricated via In Situ Etching-Growth Route.

Inorganic chemistry·2026

Same author

Exploring global research trends in sublingual immunotherapy of allergic rhinitis: a comprehensive bibliometric analysis.

Immunotherapy·2026

Same author

Dual regulatory role of Penicillium oxalicum and Lolium perenne in bauxite residue soilization: Labile organic carbon dynamics and community assembly.

Journal of environmental sciences (China)·2026

Same author

Interface engineering of fcc-RuCo@hcp-Ru core-shell nanoplates for efficient industrial alkaline hydrogen evolution.

Chemical communications (Cambridge, England)·2026

Same author

The efficacy and the safety of antenatal breastmilk expression on breastfeeding outcomes: a systematic review and meta-analysis.

BMC pregnancy and childbirth·2026

Same author

Notoginsenoside R1 as a Protector Against Gentamicin Ototoxicity: Targeting p62-NRF2-GPX4 Ferroptosis Axis.

Advanced biology·2026

Search research articles

Related Experiment Video

Updated: May 26, 2026

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Published on: May 16, 2014

Biobutanol.

Hongjun Dong¹, Wenwen Tao, Zongjie Dai

¹Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.

Advances in Biochemical Engineering/Biotechnology

|December 15, 2011

Summary

This summary is machine-generated.

China

More Related Videos

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

Biomass Conversion to Produce Hydrocarbon Liquid Fuel Via Hot-vapor Filtered Fast Pyrolysis and Catalytic Hydrotreating

Biomass Conversion to Produce Hydrocarbon Liquid Fuel Via Hot-vapor Filtered Fast Pyrolysis and Catalytic Hydrotreating

Published on: December 25, 2016

Related Experiment Videos

Last Updated: May 26, 2026

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Published on: May 16, 2014

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

Biomass Conversion to Produce Hydrocarbon Liquid Fuel Via Hot-vapor Filtered Fast Pyrolysis and Catalytic Hydrotreating

Biomass Conversion to Produce Hydrocarbon Liquid Fuel Via Hot-vapor Filtered Fast Pyrolysis and Catalytic Hydrotreating

Published on: December 25, 2016

Area of Science:

Biotechnology
Industrial Microbiology
Chemical Engineering

Background:

The acetone-butanol-ethanol (ABE) industry in China, prominent in the 1980s, declined due to petrochemical competition.
Renewed global interest in butanol as a biofuel and bio-based chemical is driven by rising oil prices and environmental concerns.
China is revitalizing its ABE industry with new plants and significant research efforts.

Purpose of the Study:

To review the recent advancements in biobutanol production in China.
To highlight progress in strain improvement and process optimization for ABE fermentation.
To provide an overview of research trends in biobutanol technology in China.

Main Methods:

Conventional mutagenesis techniques applied to enhance butanol-producing strains like Clostridium acetobutylicum.
Application of omics technologies (genomics, proteomics, transcriptomics) to understand bacterial characteristics.
Development of genetic manipulation systems, including the group II intron method, for strain engineering.

Main Results:

Improved Clostridium acetobutylicum mutant strains (e.g., EA2018 with 70% butanol ratio, Rh8 with 19 g/L tolerance) have been developed.
Omics technologies have elucidated key features of butanol-producing bacteria.
Enhanced genetic manipulation systems have led to successful engineering of production strains.

Conclusions:

China is making significant strides in biobutanol production through strain improvement and advanced biotechnological approaches.
The research encompasses both upstream (strain development) and downstream processing.
These advancements position China to contribute substantially to the global biobutanol market.