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Related Experiment Videos

Virus-like 30S RNA in mouse cells.

P Besmer, U Olshevsky, D Baltimore

    Journal of Virology
    |March 1, 1979
    PubMed
    Summary

    Mouse cells contain endogenous VL30 RNA sequences, a novel class of defective retroviruses. These sequences are rescued into virions and can be reverse transcribed, but lack biological function.

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    Area of Science:

    • Molecular Biology
    • Virology
    • Genomics

    Background:

    • Uninfected mouse cells express high levels of viral sequences.
    • BrdU-induced virus from JLS-V9 cells contains a 30S RNA genome, termed VL30 RNA.
    • VL30 RNA represents a new class of endogenous defective retroviruses.

    Purpose of the Study:

    • To characterize the nature and properties of VL30 RNA.
    • To investigate the rescue and expression of VL30 RNA in mouse cells.
    • To determine the genomic organization and prevalence of VL30 sequences.

    Main Methods:

    • Agarose gel electrophoresis to analyze RNA migration.
    • Fingerprint analysis and hybridization studies for sequence homology.
    • BrdU induction and superinfection for VL30 RNA rescue.
    • Complementary DNA probe hybridization to cellular RNA.
    • Dot blot analysis to quantify VL30 sequence copy number.

    Main Results:

    • VL30 RNA is a 30S RNA species, distinct from standard murine leukemia viruses.
    • VL30 RNA can be rescued into virions via phenotypic mixing upon BrdU induction or superinfection.
    • Rescued VL30 RNA can exist as 50S or 70S complexes and is reverse transcribed.
    • VL30 sequences are present in mouse cells (20-50 copies per genome) but not in rat or rabbit cells.
    • No subgenomic RNA related to VL30 RNA was detected in expressing cells.

    Conclusions:

    • VL30 RNA is an endogenous, defective retroviral element in mouse cells.
    • VL30 RNA can be phenotypically mixed and packaged into infectious virions.
    • VL30 RNA sequences are conserved in the mouse genome and likely present in many mouse-derived virus stocks.

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